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目的:研究某型潜艇艇员长远航前后淋巴细胞亚群、调节T淋巴细胞及相关细胞因子的变化，探讨长远航中持续应激对艇员免疫功能的影响。方法:随机抽取长远航(70 d)潜艇61名艇员为研究对象，空腹抽取静脉血，利用流式细胞仪对艇员长远航前后淋巴细胞亚群[CD3n ＋、CD4n ＋、CD8n ＋、CD4n ＋/CD8n ＋、CD3n －CD19n ＋、CD3n －CD(16＋56)n ＋]及调节T淋巴细胞(CD4n ＋CD25n ＋)进行测定，采用液态芯片技术观察白细胞介素-2(IL-2)、IL-4、IL-8、干扰素诱导蛋白-10(IP-10)含量的变化。n 结果:与长远航前相比，长远航后CD3n ＋、CD4n ＋、CD8n ＋、CD3n －CD19n ＋、CD3n －CD(16＋56)n ＋淋巴细胞水平显著降低，差异均有统计学意义(n P<0.05)；CD4n ＋/CD8n ＋的比值以及CD4n ＋CD25n ＋水平显著升高，差异均有统计学意义(n P<0.01)；IL-2、IL-8含量降低，差异均有统计学意义(n P<0.05)；IL-4、IP-10含量升高，差异均有统计学意义(n P<0.05)。n 结论:在长远航持续应激作用下，艇员的免疫功能发生明显变化，表明长远航对艇员健康有一定的影响，有必要进一步阐明原因并采取必要的预防措施。“,”Objective:To study the changes of the lymphocyte subpopulation, regulatory T cells, and related cytokines before and after long oceangoing voyage, and to explore the effect of persistent stress on immune function in submarine crew.Methods:Sixty-one submariners of long oceangoing voyage (70 d) were selected randomly. Before and after the voyage, the quantities of lymphocyte subpopulations [CD3n + , CD4n + , CD8n + , CD4n + /CD8n + , CD3n -CD19n + , CD3n -CD(16+ 56)n + ] and regulatory T cells(CD4n + CD25n + ) from peripheral blood were detected by flow cytometry; and the contents of interleukin-2(IL-2), interleukin-14(IL-14), interleukin-8(IL-8), and interferon inducible protein 10(IP-10) were detected and compared by liquid chip.n Results:Compared with those before voyage, the levels of CD3n + , CD4n + /CD8n + , CD3n -CD19n + , CD3n -CD(16+ 56)n + decreased significantly (n P<0.05), CD4n + /CD8n + and CD4n + CD25n + increased significantly(n P<0.01). The contents of IL-2 and IL-8 were lower markedly than those before voyage(n P<0.05), while the contents of IL-4 andIP-10 were higher than those before voyage (n P<0.05).n Conclusion:The immune function of submarine crew had some obvious changes under persistent stress of long oceangoing voyage, indicating long oceangoing voyage had certain effects on the health of submarine crew; which is necessary to make further research on the mechanism and take preventive measures.