论文部分内容阅读
目的:观察蝎毒多肽(PESV)对人白血病NOD/SCID小鼠髓外浸润模型体内uPA、uPAR、MMP2、MMP9表达的影响,探讨PESV干预急性白血病髓外浸润传变的机制。方法:首先选取急性白血病患者骨髓单个核细胞注入经过铯-137源照射的NOD/SCID小鼠体内,建立人白血病NOD/SCID小鼠髓外浸润模型;对实验小鼠随机分组,用Real-time PCR及Western Blot检测PESV治疗后小鼠体内MMP2、MMP9 mRNA和蛋白表达水平,用ELISA法检测小鼠血清uPA及uPAR表达水平。结果:PESV能够抑制模型小鼠体内MMP2、MMP9 mRNA及蛋白水平表达,能够抑制uPA及uPAR过度表达,其抑制效果与PESV浓度具有相关性。结论:PESV通过干预细胞外基质降解机制,阻抑急性白血病髓外浸润传变进展。
Objective: To observe the effect of PESV on the expression of uPA, uPAR, MMP2 and MMP9 in the extramedullary infiltration model of human leukemia NOD / SCID mice and to explore the mechanism of PESV intervention in the extramedullary infiltration of acute leukemia. METHODS: Bone marrow mononuclear cells from acute leukemia patients were injected into NOD / SCID mice exposed to cesium-137 source to establish the extramedullary infiltration model of human leukemia NOD / SCID mice. The mice were randomly divided into three groups: Real-time PCR and Western Blot were used to detect the expression of MMP2 and MMP9 mRNA and protein in mice after PESV treatment. The expression of uPA and uPAR in serum was detected by ELISA. Results: PESV could inhibit the expression of MMP2 and MMP9 mRNA and protein in model mice, and inhibit the overexpression of uPA and uPAR. The inhibitory effect of PESV was correlated with the concentration of PESV. Conclusion: PESV suppresses the progress of extramedullary infiltration of acute leukemia by intervening the mechanism of extracellular matrix degradation.