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目的 探讨冠心病ATP结合盒转运体G1 (ATP binding cassette transporter G1,ABCG1)基因启动子区rs1378577位点A/C多态性对血管内皮功能的影响.方法 采用聚合酶链反应-限制性片段长度多态性方法分析156例冠心病患者ABCG1基因启动子区rs1378577位点的基因型,依据基因型分为AA组、AC组和CC组,血管超声检测肱动脉血流依赖性舒张功能(FMD),生化分析仪测定血脂水平,实时荧光定量PCR检测血细胞ABCG1 mRNA的表达水平.结果 与AA组ABCG1 mRNA/GAPDH比值(0.278±0.009)相比,AC组比值(0.255±0.010)降低(P＜0.01),CC组比值(0.257±0.008)降低(P＜0.01).与AA组(11.115±0.283)％相比,AC组(9.018±0.233)％降低(P＜0.01),CC组(8.976±0.301)％降低(P＜0.01).ABCG1 mRNA/GAPDH比值与FMD相关性分析,AA组呈正相关(r=0.953,P＜0.01),AC组呈正相关(r=0.867,P＜0.01),CC组呈正相关(r=0.813,P＜0.01).结论 AA基因型相比,冠心病患者ABCG1基因启动子区rs1378577位点AC和CC基因型其血管内皮功能下降,与ABCG1 mRNA表达下降有关.“,”Objective To address the effect of rs1378577 A/C polymorphism in ATP binding cassette transporter Gl (ABCG1) promoter region on vascular endothelial function in patients with coronary heart disease.Methods 156 patients with coronary heart disease were grouped into AA,AC and CC based on their genotype of rs1378577 in ABCG1 promoter region determined by polymerase chain reaction-restricted fragment length polymorphism.Blood lipid level was measured by biochemical analyzer.The ABCG1 mRNA expression was detected by real-time fluorescence quantitative PCR.The flow mediated dilatation (FMD) function in brachial artery was measured by high-resolution ultrasound.Results The ratios of ABCG1 mRNA/GAPDH in both group AC and CC were significantly lower as compared with group AA (0.255 ±0.010 vs 0.278±0.009;0.257±0.008 vs 0.278±0.009,P＜0.01).And compared with AA group,the values of FMD in both group AC and CC were much lower,the differences were statistically significant (11.115％±0.238％ vs 9.018％±0.233 ％;11.115 ％ ± 0.238 ％ vs 8.976 ％ ± 0.301％,P＜0.01).The correlation analysis showed that ABCG1 mRNA/GAPDH ratio and FMD were positively correlated in AA group (r=0.953,P＜0.01),AC group (r =0.867,P＜0.01) as well as in CC group (r=0.813,P＜0.01).Conclusions The vascular endothelial function of AC and CC genotype in rs1378577 of ABCG1 promoter in patients with coronary heart disease weakens which is associated with the decreased expression of ABCG1 mRNA.