论文部分内容阅读
目的研究血小板衍生生长因子在病毒性肝炎肝纤维化中的作用。方法以免疫组化技术检测血小板衍生生长因子（ＰＤＧＦ）－ＢＢ以及ＰＤＧＦ的β受体（ＰＤＧＦＲ－β）在病毒性肝炎肝组织中的表达。结果ＰＤＧＦ－ＢＢ及ＰＤＧＦＲ－β在肝组织中的表达与肝纤维化程度明显相关，肝硬变及慢性肝炎Ｓ３～４期患者肝组织ＰＤＧＦ－ＢＢ及ＰＤＧＦＲ－β表达强度明显高于急性肝炎及慢性肝炎Ｓ０～２期患者，同时与结蛋白、Ⅲ型前胶原肽在肝组织中的表达以及血清金属蛋白酶组织抑制因子－１（ＴＩＭＰ－１）呈明显正相关。结论ＰＤＧＦ－ＢＢ及ＰＤＧＦＲ－β不仅对星状细胞的活化、分裂、增殖及其合成胶原等细胞外基质（ＥＣＭ）均有明显的促进作用，而且可能通过上调ＴＩＭＰ－１减少ＥＣＭ的降解，促进肝纤维化的发生和进展过程。
Objective To study the role of platelet-derived growth factor in liver fibrosis induced by viral hepatitis. Methods The expression of platelet derived growth factor (PDGF) -BB and PDGF receptor β (PDGFR-β) in viral hepatitis liver tissue were detected by immunohistochemistry. Results The expressions of PDGF-BB and PDGFR-β in liver tissues were significantly correlated with the degree of liver fibrosis. The expressions of PDGF-BB and PDGFR-β in cirrhotic and chronic hepatitis patients with stage S3 ~ 4 were significantly higher than those with acute hepatitis and Chronic hepatitis S0 ~ 2 patients, at the same time with desmin, type Ⅲ procollagen peptide expression in the liver and serum tissue inhibitor of metalloproteinase-1 (TIMP-1) was positively correlated. Conclusion PDGF-BB and PDGFR-β not only promote the activation, division, proliferation and extracellular matrix (ECM) of stellate cells, but also promote the degradation of ECM by up-regulating TIMP-1 The occurrence and progression of liver fibrosis.