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目的探讨妊娠期糖尿病母亲新生儿T细胞亚群及NK细胞活性的变化及其与患儿出生后24 h内微量血糖的关系。方法选择妊娠期糖尿病母亲足月新生儿33例(观察组)及健康足月新生儿30例(对照组),应用流式细胞术检测出生后24 h内静脉血CD3+、CD4+、CD8+T细胞及CD16+CD56+NK细胞;采用血糖检测仪检测新生儿生后24 h内的微量血糖,并研究观察组血糖与CD3+、CD4+、CD8+5T细胞、CD4+/CD8+、CD16+CD56+NK细胞有无相关性。结果观察组新生儿的CD3+,CD4+T细胞计数,CD4+/CD8+比值、CD16+CD56+NK细胞活性均低于对照组新生儿(P<0.01),微量血糖亦低于对照组(P<0.05),观察组低血糖发生率21.21%,对照组无低血糖发生,两组低血糖发生率差异有统计学意义(P=0.023)。CD3+、CD4+T细胞计数,CD16+CD56+NK细胞活性与微量血糖呈正相关性(P均<0.05)。结论妊娠期糖尿病可导致新生儿CD3+、CD4+T细胞计数,CD4+/CD8+比值,CD16+CD56+NK细胞活性下降,导致其免疫功能下降,应加强其围产期护理,预防新生儿期感染的发生。
Objective To investigate the changes of neonatal T cell subpopulation and NK cell activity in gestational diabetic mothers and their relationship with the level of trace glucose within 24 h after birth. Methods Thirty-three neonates with full-term gestational diabetes mellitus (DM group) and 30 healthy term neonates (control group) were selected. The levels of CD3 +, CD4 +, CD8 + T cells in venous blood were measured by flow cytometry And CD16 + CD56 + NK cells. The blood sugar level was measured within 24 hours after birth, and the levels of blood glucose and CD3 +, CD4 +, CD8 + 5T cells, CD4 + / CD8 +, CD16 + CD56 + NK cells No correlation. Results The neonatal neonates had lower levels of CD3 +, CD4 + T cells, CD4 + / CD8 + ratio and CD16 + CD56 + NK cell activity than those of the control group (P <0.01) ). The incidence of hypoglycemia was 21.21% in the observation group and no hypoglycemia in the control group. There was significant difference in the incidence of hypoglycemia between the two groups (P = 0.023). CD3 +, CD4 + T cell count and CD16 + CD56 + NK cell activity were positively correlated with micro-glucose (P <0.05). Conclusions Gestational diabetes mellitus can lead to the decrease of neonatal CD3 +, CD4 + T cell count, CD4 + / CD8 + ratio and CD16 + CD56 + NK cell activity, resulting in decreased immune function. occur.