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目的运用聚合酶链反应技术结合常规法的检测手段,提高外环境霍乱弧菌的阳性检出率。方法通过聚合酶链反应技术和常规技术相结合的综合检测方法与单纯的常规技术的检测方法进行比较,观察分析其阳性检出率。结果聚合酶链反应技术和常规技术相结合的综合检测技术,其阳性检出率(0.3%)高于单纯的常规检测技术的阳性检出率(0.2%)。结论运用聚合酶链反应技术结合常规法进行霍乱弧菌的检测,可提高其阳性检出率,同时又能减轻工作量,提高工作效率。
Objective To improve the detection rate of Vibrio cholerae in the external environment by polymerase chain reaction (PCR) combined with routine detection. Methods The method of polymerase chain reaction (PCR) and conventional techniques were compared with those of simple routine techniques, and the positive detection rate was observed and analyzed. Results The positive detection rate (0.3%) was higher than that of simple routine detection (0.2%) by the combination of polymerase chain reaction and routine techniques. Conclusion The detection of Vibrio cholerae by polymerase chain reaction and conventional methods can increase the detection rate of Vibrio cholerae while improving the work efficiency and reducing the workload.