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用超临界CO2流体萃取技术从曼地亚红豆杉枝条中提取紫杉醇。在分离釜Ⅰ和Ⅱ温度为30℃、压力为6.5MPa、CO2体积流量为25L/h条件下,正交实验优化所得萃取过程的最佳工艺参数为:萃取时间3.5h;萃取压力30MPa;萃取温度47℃;夹带剂为φ(CH3CH2OH)=85的乙醇溶液。萃取方式为静态和动态萃取相结合。最佳工艺条件下各用35.0g原料进行3次验证实验,紫杉醇的平均提取量为9.58mg,平均提取率87.76;浸膏中紫杉醇质量分数为1.12,高于传统的溶剂浸提(浸膏中紫杉醇的质量分数约0.87)。
Taxol was extracted from the branches of Taxus chinensis by supercritical CO2 fluid extraction. Under the conditions of 30 ℃, 6.5 MPa and CO2 volume flow of 25 L / h, the optimum process parameters of orthogonal experiment were as follows: extraction time 3.5 h; extraction pressure 30 MPa; extraction Temperature 47 ℃; entrainer φ (CH3CH2OH) = 85 ethanol solution. Extraction method for the combination of static and dynamic extraction. Under optimum conditions, 35.0g raw materials were used for 3 times of validation experiments. The average extraction yield of paclitaxel was 9.58mg and the average extraction rate was 87.76. The content of paclitaxel in the extract was 1.12, higher than the traditional solvent extraction Paclitaxel mass fraction of about 0.87).