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用低能N+离子束对香瓜种子进行不同能量和剂量组合的处理。从各处理组与对照组当代和第三代苗期形态特征观察、考种中发现 :在一定能量和剂量组合下 ,处理组香瓜叶型和果肉厚度发生了明显的变化 ,这种形态学变化是可遗传的。应用随机引物扩增多态性DNA (RandomamplifiedpolymorphicDNA即RAPD)技术分析对照组和注入低能N+离子束处理组的香瓜总DNA的结果显示 :10 0种随机引物中的 2 4种引物扩增出 44条多态性片段 ,且其中一种引物所扩增的条带与叶型变化相连锁。表明低能N+注入香瓜种子可引起其基因组DNA发生变异 ,一定注入剂量和能量的组合 ,可导致特异性的变异。
Muskmelon seeds were treated with different energy and dose combinations with low energy N + ion beam. Observed from the morphological characteristics of the seedlings of the contemporary and the third generation in each treatment group and the control group, it was found in the test species that the leaf thickness and the thickness of the pulp of the melon under the certain energy and dose combinations changed significantly, and this morphological change Is heritable The results of random amplified polymorphic DNA (RAPD) analysis of the control group and the total DNA of Muskmelon injected with low energy N + ion beam showed that 44 of the 104 kinds of random primers amplified 44 Polymorphic fragments, and one of the primer amplified bands linked to leaf type changes. It shows that the low energy N + injection of melon seeds can lead to the mutation of genomic DNA, and the combination of dose and energy must be injected to cause specific mutation.