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目的:研究甲钴胺(methylcobalamin,Methyl B12)对损伤背根节(dorsal root ganglion,DRG)A-类神经元的不同模式自发放电的作用。方法:在大鼠L5椎间孔植入不锈钢柱制备大鼠DRG慢性压迫模型(chronic com-pression of DRG,CCD)上,利用在体单纤维细胞外记录方法研究Methyl B12对受损DRG神经元自发放电的影响。结果:(1)CCD模型大鼠出现明显的双侧机械刺激引起缩足反应阈值降低(P<0.001);(2)Methyl B12(300μmol/L)对受损A-类DRG神经元自发放电具有显著的抑制作用(n=13,P<0.05);(3)Methyl B12(300μmol/L)对受损A-类DRG神经元非周期和周期放电模式自发放电均有抑制作用,且两者之间没有明显差异。结论:Methyl B12(300μmol/L)对受损DRG A-类神经元产生的异位自发放电具有抑制作用,且对于非周期和周期放电模式的抑制作用没有差异。
Objective: To investigate the effect of methylcobalamin (Methyl B12) on spontaneous firing in different modes of dorsal root ganglion (DRG) type A neurons. Methods: Methyl B12 was used to study the damage of DRG neurons in chronic com-pression of DRG (CCD) in rat L5 intervertebral foramen implanted with stainless steel column. The impact of spontaneous discharge. (2) Methyl B12 (300μmol / L) had a significant effect on the spontaneous discharge of damaged A-like DRG neurons with (P <0.001) (3) Methyl B12 (300μmol / L) inhibited the spontaneous firing in both non-periodic and periodic discharge patterns in damaged A-type DRG neurons, and the inhibitory effect of both There is no significant difference between. CONCLUSION: Methyl B12 (300 μmol / L) has an inhibitory effect on ectopic spontaneous discharge generated by damaged DRG A-type neurons, and there is no difference in the inhibition of aperiodic and periodic discharge patterns.