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Intense 2.7-\mu m emissions are obtained from Er3 /Nd3 co-doped tellurite glass samples under the 808-nm laser diode excitation. According to the absorption spectra, Judd-Ofelt parameters and radiative transition probabilities are calculated and analyzed using the Judd-Ofelt theory. The spectroscopic properties and energy transfer mechanism between Er3 and Nd3 are analyzed. The effects of OH content on the spectroscopic properties of Er3 /Nd3 co-doped samples are discussed. The obtained results indicate that Er3 /Nd3 co-doped tellurite glass can significantly develop optical properties of 2.7-\mu m emission, if OH groups can be effectively eliminated.