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目的:探讨TGF-β1对卵巢癌细胞A2780增殖、迁移及侵袭能力的影响。方法:以体外培养的卵巢癌细胞A2780为研究对象,给予不同浓度(0、2、4…20 ng/m L)TGF-β1处理不同时间(12、24…72 h)。采用CCK-8法检测不同的浓度TGF-β1处理不同时间对卵巢癌细胞A2780增殖的影响。根据增殖实验结果选择合适的TGF-β1作用浓度及处理时间,采用细胞划痕实验测定细胞的迁移能力,Transwell实验检测细胞的侵袭及迁移能力。结果:相较于空白对照组,TGF-β1可以剂量和时间依赖性显著促进卵巢癌细胞A2780的增殖(P<0.05)。细胞划痕实验结果显示TGF-β1处理组ΔS%/h明显高于空白对照组(P<0.05);Transwell迁移实验结果显示:TGF-β1处理组OD570明显高于空白对照组,差异具有统计学意义(P<0.05)。Transwell细胞侵袭实验结果显示:与空白对照组相比,TGF-β1处理组OD570明显升高(P<0.05)。结论:TGF-β1可以明显促进卵巢癌细胞系A2780的增殖、迁移及侵袭能力,其促增殖效应呈剂量/时间依赖效应。
Objective: To investigate the effect of TGF-β1 on proliferation, migration and invasion of ovarian cancer cell line A2780. METHODS: A2780 ovarian cancer cells cultured in vitro were treated with TGF-β1 at different concentrations (0, 2, 4 ... 20 ng / m L) for different times (12,24 ... 72 h). The effects of different concentrations of TGF-β1 on the proliferation of ovarian cancer cell line A2780 were detected by CCK-8 assay. According to the result of proliferation experiment, the appropriate concentration of TGF-β1 and the treatment time were selected. The cell migration ability was determined by cell scratch assay. The invasion and migration ability of cells was detected by Transwell assay. Results: Compared with the blank control group, TGF-β1 significantly promoted the proliferation of ovarian cancer cell A2780 in a dose-and-time-dependent manner (P <0.05). Cell scratch assay showed that ΔS% / h in TGF-β1-treated group was significantly higher than that in blank control group (P <0.05). Transwell migration assay showed that OD570 in TGF-β1-treated group was significantly higher than that in blank control group Significance (P <0.05). Transwell cell invasion assay showed that compared with the blank control group, the OD570 of TGF-β1-treated group was significantly increased (P <0.05). Conclusion: TGF-β1 can significantly promote the proliferation, migration and invasion of ovarian cancer cell line A2780 in a time-and dose-dependent manner.