为探讨HFRSV在螨体组织细胞内的定位 ,采用螨单层细胞培养观察HFRSV。结果发现 :2 4小时后螨单层细胞生长良好 ,15天生长旺盛 ,2 0天有少量细胞病变、脱落 ;15天和2 0天可从贴壁细胞检测出HFRSV。经PCR扩增鉴定 ,革螨和恙螨所携带的HFRSV其抗原性一致。表明革螨、恙螨体内有HFRSV存在 To investigate the localization of HFRSV in mite cells, HFRSV was observed by mite monolayer culture. The results showed that, after 24 hours, the mite monolayer cells grew well, grew vigorously on the 15th day, had few cytopathic lesions on day 20, and shed off; on days 15 and 20, HFRSV was detected from adherent cells. According to the PCR amplification, the antigenicity of HFRSV in leather mites and chigger mites was the same. Show leather mites, chigger mites HFRSV exist in vivo