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目的检测Ca(OH)2对体外培养的人牙髓细胞内Ca2+的影响。方法在RPMI1640培养液中加入Ca(OH)2、CaCl2和NaHCO4,培养第6代人牙髓细胞，用Fluo-3荧光探针负载后，再次用Ca(OH)2刺激，激光扫描共聚焦显微镜检测细胞内Ca2+。结果含Ca(OH)2培养液培养的牙髓细胞，再用Ca(OH)2刺激时胞内Ca2+升高；其他条件培养的细胞，Ca(OH)2刺激时Ca2+无变化。结论Ca(OH)2可导致人牙髓细胞内Ca2+升高。“,”Objective To investigate the influence of calcium hydroxide to intracellular calcium ion of human pulp cells in vitro. Methods The sixth generation of human pulp cells were maintained in RPMI 1640 medium supplemented with Fluo-3 probe and Ca(OH)2 for restimulation. The intracellular calcium ion concentration was detected by laser confocal microscope. Results The intracellular calcium ion level in Ca(OH)2 pretreatment group increased after Ca(OH)2 stimulation,while it didn′t response to Ca(OH)2 stimulus in group without Ca(OH)2、CaCl2 or NaHCO4 pretreatment. Conclusion It is important to pretreat the pulp cells with Ca(OH)2 in order to increase the intracellular calcium.