Based on the land use change data obtained from the interpretation of remote sensing images in 1985, 1995, 2005 and 2008, the research applied principal component analysis, Markov chain and ESDA method, and took cultivated land patch as the basic unit of landscape. A comprehensive measure of the fragmentation of cultivated land was made in Jiangsu counties and its temporal and spatial evolution characteristics and driving process from the perspective of landscape ecology were discussed. The results are as follows:(1)The overall fragmentation of cultivated land in Jiangsu Province presents that the eastern coastal areas are low, the interior of the west is high, the fragmentation of northern and southern Jiangsu is high, while the fragmentation of mid-Jiangsu is low.(2)The degree of fragmentation of cultivated land in most area of southern Jiangsu and midJiangsu showed an upward trend, while the fragmentation of cultivated land in northern Jiangsu showed a downward trend from 1985 to 2008. The degree of agglomeration of cultivated land is weakened continuously, the area of high value agglomeration is decreasing and the trend of “polarization” is weakening.(3) Topographic condition is the decisive factor for the formation of the difference pattern. The change of rural residential land, cultivated land and the scale of urban land use plays a role on the cultivated land fragmentation index, among them, the change of rural residential land scale has the most significant effect on cultivated land fragmentation. Based on the land use change data obtained from the interpretation of remote sensing images in 1985, 1995, 2005 and 2008, the research applied principal component analysis, Markov chain and ESDA method, and taken cultivated land patch as the basic unit of landscape. A comprehensive measure of the fragmentation of cultivated land was made in Jiangsu counties and its temporal and spatial evolution characteristics and driving process from the perspective of landscape ecology were discussed. presents that the eastern coastal areas are low, the interior of the west is high, the fragmentation of northern and southern Jiangsu is high, while the fragmentation of mid-Jiangsu is low. (2) The degree of fragmentation of cultivated land in most area of southern Jiangsu and midJiangsu showed an upward trend, while the fragmentation of cultivated land in northern Jiangsu showed a downward trend from 1985 to 20 08. The degree of agglomeration of cultivated land is weakened continuously, the area of ​​high value agglomeration is decreasing and the trend of “polarization ” is weakening. (3) Topographic condition is the decisive factor for the formation of the difference pattern. The change of rural residential land, cultivated land and the scale of urban land use plays a role on the cultivated land fragmentation index, among them, the change of rural residential land scale has the most significant effect on cultivated land fragmentation.