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目的 探讨瘤内注射复方中药 99 克星治疗肝细胞癌的抗癌作用及其机制。方法 取人肝癌SMMC 772 1裸鼠皮下移植与肝内原位移植模型各 16只 ,随机分为 99 克星治疗组 16只 ,无水乙醇与生理盐水治疗组各 8只。全部裸鼠在接种 10d后每隔 5d向肿瘤内注入治疗药物共 4次 ,治疗 2 0d后处死 ,取肿瘤组织分别行病理光镜、电镜检查及流式细胞 (FCM )DNA分析。治疗前后分别采用高频超声测量肿瘤的三径并计算肿瘤的生长指数 [(治疗后肿瘤体积 -治疗前肿瘤体积 ) /治疗前肿瘤体积 ]。所有实验均在双盲条件下进行。结果 克星组肿瘤生长指数 (0 .0 68± 0 .0 2 2 )明显低于盐水组 (4 .3 5 4± 1.45 3 ) (P <0 .0 1) ,克星组肿瘤生长指数虽也低于乙醇组 (0 .0 79± 0 .0 2 4) ,但差异无显著性意义 (P >0 .0 5 )。病理见盐水组药物影响或破坏肿瘤作用轻微 ;乙醇组肿瘤组织以凝固性坏死为主 ;克星组则以肿胀变性坏死为主伴大量淋巴细胞浸润 ,电镜发现淋巴细胞明显攻击癌细胞现象 ,多数可见典型凋亡细胞或 (和 )凋亡小体与成簇状排列的胶原纤维。流式细胞 (FCM )DNA分析显示克星组凋亡峰出现率高达 93 .8% ,明显高于乙醇组与盐水组的 12 .5 % (P <0 .0 1)。结论 99 克星具有强大的抑制与破坏肿瘤细胞生长的作用 ,其疗效
Objective To investigate the anticancer effect and mechanism of intratumor injection of 99 g Chinese herbal medicine on hepatocellular carcinoma. Methods Twenty - six human hepatocellular carcinoma SMMC 772 1 xenografts and orthotopic liver transplantation were randomly divided into four groups (n = 99) and control group (n = 16). Eight rats in each group were treated with ethanol and saline. All nude mice were injected with the drug four times every 5 days after inoculation for 10 days. After 20 days of treatment, they were sacrificed and the tumor tissues were taken out for pathological light microscopy, electron microscopy and flow cytometry (FCM) DNA analysis. Before and after treatment, the three diameters of tumor were measured by high frequency ultrasound and the growth index of tumor was calculated [(tumor volume after treatment - tumor volume before treatment) / tumor volume before treatment]. All experiments were performed under double-blind conditions. Results The tumor growth index of nemesis group (0.068 ± 0.222) was significantly lower than that of saline group (4.34 ± 1.453) (P <0.01) In alcohol group (0.079 ± 0.024), but the difference was not significant (P> 0.05). Pathological see saline group of drugs affect or destroy the role of the tumor slightly; ethanol group of tumor coagulation necrosis; nemesis group is swollen degeneration and necrosis accompanied by a large number of lymphocytic infiltration, electron microscopy found that lymphocytes significantly attack the cancer cells, the majority of visible Typical apoptotic cells or (and) apoptotic bodies and clustered collagen fibers. Flow cytometry (FCM) DNA analysis showed that the rate of apoptosis peak in nemesis was as high as 93.8%, which was significantly higher than that in ethanol group and saline group (P <0.01). Conclusion 99 grams of star has a strong inhibition and destruction of tumor cell growth, its efficacy