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目的：探讨补肾生精汤对铅损伤小鼠生精细胞凋亡的影响，为临床治疗重金属所致男性不育提供理论依据。方法成年健康清洁级ICR雄性小鼠30只，随机分为对照组、模型组和给药组，每组10只。醋酸铅灌胃建立睾丸损伤模型（30mg/kg体质量，0.1ml/10g容积），采用中药补肾生精汤灌胃治疗（15.0g/kg，即25mL/kg体质量），每天1次，共28d。TUNEL法检测细胞凋亡情况，共聚焦激光扫描显微镜观察；JC-1染色法检测线粒体膜电位变化情况，NucleoCounter NC-3000TM对线粒体膜电位进行分析。结果 TUNEL法检测，模型组凋亡细胞数量（11.60±2.01）明显高于对照组（1.20±1.03）（P ＜0.01），阳性细胞多发生于精原细胞和精母细胞的位置；给药组凋亡细胞数（3.40±1.78）明显低于模型组（P ＜0.01）。JC-1染色法检测，模型组去极化细胞（凋亡细胞）比例为（74.20%±1.3038），明显高于对照组（31.80%±0.8367）（P ＜0.01）；给药组去极化细胞比例为（54.20%±1.3038），低于模型组（P＜0.01）。结论补肾生精汤可通过抑制生精细胞凋亡而改善铅损伤小鼠的生精功能。“,”Objective To investigate the effects of Bu Shen Sheng Jing Tang on apoptosis of spermatogenic cells in lead-treated mice, and establish the theoretical basis for clinical treatment of male infertility induced by heavy metals . Methods Total of 30 adult male ICR mice were randomly divided into three groups such as the control group, the model group and the treated group. Testis-injuried model was made by intragastric administration of lead acetate(30mg?kg-1, 0.1ml?10g-1). Mice in the treated group received intragastric administration of lead acetate and Bu Shen Sheng Jing Tang (15.0g?kg-1, 25mL?kg-1) at the same time for 28days. Mice in the control group received the same amount of 0.9% NaCl by intragastric administration as well. Apoptosis was examined with TUNEL and observed under the confocal laser scanning microscope. Mitochondrial potential was examined with JC-1 and analyzed by NucleoCounter NC-3000TM. Results The number of apoptosis cells(3.40±1.78) in the treated group was lower than that in the model group(11.60±2.01)(P<0.01), moreover the number of apoptosis cells of these two groups were all higher than that of the control group. The rate of depolarization cells(apoptosis cells) (54.20%±1.3038) was lower than that in the model group (74.20%±1.3038) (P<0.01). Conclusion The results suggest that Bu Shen Sheng Jing Tang supplementation protects the testicular spermatogenisis of lead-treated mice by inhibiting spermatogenic cells apoptosis.