【摘 要】
:
以大豆重组自交系soy01群体中的255个家系为作图群体,利用225个分子标记和2个形态标记构建了一张包含27个连锁群的大豆遗传连锁图谱,总遗传距离1 315.46 cM,平均标记间距5.79
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以大豆重组自交系soy01群体中的255个家系为作图群体,利用225个分子标记和2个形态标记构建了一张包含27个连锁群的大豆遗传连锁图谱,总遗传距离1 315.46 cM,平均标记间距5.79 cM。在构建遗传图谱的基础上,采用复合区间作图法,检测到10个与含油量相关的QTL。这些QTL分别位于大豆遗传图谱的A2、C2、D1b、M和N连锁群,解释的遗传变异范围为4.0%~12.2%。这些QTL中,4个与Soybase数据库中的QTL有可能是相同的位点,2个可能是新的位点,其余4个是否是新的位点还有待进一步验证。亲本中豆29和中豆32中均有增效基因分布,通过遗传重组可以提高大豆含油量。
Using 255 molecular markers and 2 morphological markers as markers, 255 genetic lines from soybean inbred line soybean01 were used to construct a genetic linkage map of soybean with 27 linkage groups with a total genetic distance of 1 315.46 cM, The average mark spacing is 5.79 cM. Based on the constructed genetic map, 10 QTLs related to oil content were detected by compound interval mapping. These QTLs were located in linkage groups A2, C2, D1b, M and N of the soybean genetic map, explaining genetic variation ranging from 4.0% to 12.2%. Of these QTLs, four were likely to be the same loci as the QTLs in the Soybase database, two were likely to be new loci and the remaining four were new loci for further validation. Parents 29 and Zhongdou 32 have synergistic gene distribution, genetic recombination can increase soybean oil content.
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