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对水稻类病变坏死突变体lmm1(LmmKaty)与原亲本及双单倍体YT14和YT16接种稻瘟病菌24h内的抗病反应、细胞学和分子作用机理进行了研究。人工接种实验表明,与原亲本相比,该突变体对稻瘟病菌具有更强的抗性。突变体接种20～24h后,自动荧光检测到细胞死亡,而对照则未检测到。表明该突变体通过细胞程序性死亡阻止稻瘟病菌的蔓延,从而提高了抗病性。Northernblotting结果发现,抗病品系YT14中的防卫相关基因、苯丙氨酸解氨酶基因和β-葡聚糖酶基因在人工接种稻瘟病菌6h后开始表达,16～24h后上述基因表达量快速增加;而病程相关蛋白基因PR-1和几丁质酶基因在人工接种稻瘟病菌24h后才开始表达。与YT14相比,感病品系中上述基因的表达明显延迟。表明在人工接种稻瘟病菌24h内水稻可启动抗病防卫反应,从而对稻瘟病菌产生抗性。
The disease resistance, cytology and molecular mechanism of rice blast disease mutant lmm1 (LmmKaty) and the original parents and the double haploid YT14 and YT16 inoculated with M. grisea within 24h were studied. Artificial inoculation experiments showed that the mutant was more resistant to Magnaporthe grisea than the original parent. Mutant inoculation 20 ~ 24h, autofluorescence detected cell death, while the control was not detected. This mutant showed increased disease resistance by preventing programmed cell death from blast fungus. Northern blotting showed that the defense-related genes, phenylalanine ammonia-lyase gene and β-glucanase genes of resistant strain YT14 began to be expressed 6 h after artificial inoculation with M. grisea. After 16 ~ 24 h, the above genes were rapidly expressed While PR-1 and chitinase genes of pathogenicity-related proteins began to be expressed after artificial inoculation of Magnaporthe grisea for 24h. Compared with YT14, the expression of the above genes in the susceptible lines was significantly delayed. The results showed that rice could start the disease-resistant defense response within 24 hours of inoculation of Magnaporthe grisea and thus produce resistance to Magnaporthe grisea.