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在水培条件下,以辽春10号小麦品种为供试材料,研究了10-4mol.L-1、10-5mol.L-1、10-6mol.L-13种不同浓度的水杨酸(SA)对1mmol.L-1Cd2+、5mmol.L-1Zn2+复合胁迫下小麦幼苗生长及生理特性的影响。结果表明:与对照组(未加SA处理的Cd2+、Zn2+复合污染)相比,经SA处理Cd2+、Zn2+复合胁迫下小麦幼苗地上及地下生物量都有明显增高,特别是10-4mol.L-1SA处理组分别增加23.78%和24.7%;10-4mol.L-1SA处理组小麦幼苗根系活力提高6.74%。在试验第6 d、11 d根系和叶片中超氧化物歧化酶(SOD)、过氧化物酶(POD)分别达到峰值且以10-4mol.L-1SA处理组最高;在整个试验期间3个SA处理组过氧化氢酶(CAT)活性始终高于对照,其中10-4mol.L-1SA处理组为对照组的146.6%;SA处理使丙二醛(MDA)含量降低,在试验第11 d,10-4mol.L-1SA处理组叶片和根系中丙二醛含量分别为对照组的85.5%和87.6%。但是在试验第14 d各组小麦幼苗叶片和根系中丙二醛含量全部有所回升。综合来看,不同浓度的SA处理均能提高小麦幼苗的抗氧化酶活性且高浓度SA(10-4mol.L-1)处理效果最明显。这表明,水杨酸能够通过促进保护酶活性的升高,抑制活性氧含量的增加,从而有效缓解Cd2+、Zn2+复合胁迫对小麦的毒害。
Under hydroponic conditions, wheat varieties Liaocheng 10 were used as materials to study the effects of 10-4mol.L-1,10-5mol.L-1,10-6mol.L-13 different concentrations of salicylic acid (SA) on the growth and physiological characteristics of wheat seedlings under the combined stress of 1mmol.L-1Cd2 +, 5mmol.L-1Zn2 +. The results showed that aboveground and underground biomass of wheat seedlings increased significantly under Cd2 + and Zn2 + combined treatment with SA, compared with the control (SA2 +, Cd2 +, Zn2 + combined pollution), especially 10-4mol.L- 1SA treatment increased 23.78% and 24.7% respectively. The root activity of wheat seedlings increased by 6.74% in 10-4mol.L-1SA treatment group. At the 6th, 11th day, the superoxide dismutase (SOD) and peroxidase (POD) in roots and leaves peaked and peaked at 10-4mol.L-1SA, respectively. During the whole experiment, three SA The activity of catalase (CAT) in treatment group was always higher than that in control group, of which, 10.6 mol.L-1SA treatment group was 146.6% of the control group; SA treatment reduced the content of malondialdehyde (MDA) The content of malondialdehyde in leaves and roots of 10-4mol.L-1SA treatment group were 85.5% and 87.6% of the control group respectively. However, the content of malondialdehyde in the leaves and roots of wheat seedlings all increased on the 14th day. Taken together, different concentrations of SA could improve the antioxidant enzyme activity of wheat seedlings and the highest concentration of SA (10-4mol.L-1) treatment effect most obvious. This indicates that salicylic acid can effectively alleviate the toxicity of Cd2 + and Zn2 + to wheat by promoting the increase of protective enzyme activity and inhibiting the increase of reactive oxygen species.