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目的:研究肺内原位细胞间隙连接通讯(GJIC)的受阻与肺肿瘤发生之间的关系及其防癌应用。方法:4周龄昆明小鼠肺癌变以乌拉坦启动,焦亚硫酸钠促进。部分致癌动物于启动前/后用若干可能促进细胞连接通讯的天然物/提取物进行干预。8～12周龄时用微注射荧光示踪法检测各组部分动物新鲜肺标本内原位细胞连接通讯。22周龄时所有动物作肺肿瘤检查。发生率和多发性(x-±s)的组间比较分别用χ2和双侧t检验。结果:早在肺肿瘤未出现前致癌启动+促进(乌拉坦+焦亚硫酸钠)组肺内原位GJIC严重受阻的发生率(87.5%)即已极显著高于正常组(12.5%,P=0.005)。其后前者肺肿瘤发生率(89.5%)也极显著高于后者的自发肺瘤发生率(15.0%,P<0.001)。致癌启动后饮食补充绿茶+大枣使肺内原位GJIC严重受阻发生率及肺腺瘤发生率分别降至12.5%和50.0%,均极显著低于(P<0.01)其相应的(致癌启动+促进)对照组。启动前后饮用0.5%牛磺酸34d的动物其肺内该通讯严重受阻发生数和肺腺瘤多发性分别从其对照组(乌拉坦)的1.63±1.19和1.37±1.26降至0.38±0.74(P<0.05)和0.52±0.81(P<0.02)。结论:小鼠肺内原位细胞连接通讯受阻与肺肿瘤的发生成因果关系。新鲜肺标本内这种通讯的测定可成为筛选有效防肺癌药物/保健品的特异新技术。
OBJECTIVE: To study the relationship between the obstruction of in situ interstitial cell gap junctions (GJIC) and the development of lung neoplasms and its anti-cancer application. Methods: Four-week-old Kunming mice with lung cancer started urethane and sodium metabisulfite. Some of the oncogenic animals intervene with a number of natives / extracts that may facilitate cell-ligation communication before and after priming. At 8-12 weeks of age, microinjection fluorescence tracer method was used to detect the in situ cell ligation in fresh lung specimens of some animals in each group. Lung tumors were examined at 22 weeks of age in all animals. Incidence and multiple (x- ± s) comparisons between groups were used χ2 and two-tailed t-test. Results: The incidence of in-situ GJIC in the lung (87.5%) was significantly higher than that in the normal group (12.5%, P = 0.005) as early as before carcinogenesis and promotion of lung cancer (urethane + sodium metabisulfite) ). The incidence of lung tumors in the former group (89.5%) was also significantly higher than the latter (15.0%, P <0.001). The incidence of severe in-situ GJIC obstruction and the incidence of pulmonary adenoma in the lung after diet-supplemented green tea and jujube started to decrease to 12.5% and 50.0%, respectively, after the initiation of cancer (P <0.01) + Promotion) control group. Animals that consumed 0.5% taurine for 34 days before and after initiation had a significant reduction in the number of pulmonary obstruction and pulmonary adenomas in their lungs from 1.63 ± 1.19 and 1.37 ± 1.26 in their control group (urethane) to 0.38 ± 0.74 (P <0.05) and 0.52 ± 0.81 (P <0.02). Conclusion: There is a causal relationship between the obstruction of in situ cellular junctions and the occurrence of lung neoplasms in mice. Determination of this communication in fresh lung specimens can be a novel and specific technique for screening effective anti-lung cancer drugs / supplements.