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目的观察颈静脉注射纳米金颗粒对大鼠血脑屏障结构的影响。方法经颈静脉向大鼠注射50 nm金颗粒。分别在1、2、3、4、5、6 h时收集大鼠脑脊液,采用电感偶合等离子体质谱仪检测脑脊液纳米金颗粒浓度。在注射纳米金颗粒6 h后取脑组织并固定制片,在透射电镜下观察纳米金颗粒及脑组织的超微结构;大鼠脑组织制作切片后经普鲁士蓝染色、免疫组织化学染色和免疫荧光染色,在光镜下观察大鼠血脑屏障的出血情况和完整性。结果大鼠脑脊液中纳米金颗粒浓度随时间延长逐渐升高,并在3 h后趋于稳定;电镜下可见大鼠脑细胞布满纳米金颗粒,超显微结构有一定改变;光镜下,大鼠血脑屏障在普鲁士蓝染色后可见出血点,在免疫组织化学染色和免疫荧光染色后可见血管缺损。结论纳米金颗粒可透过血脑屏障并造成微小出血点及血管微小缺损。
Objective To observe the effect of nano-gold particles injected into jugular vein on the structure of blood-brain barrier in rats. Methods Rats were injected with 50 nm gold particles through the jugular vein. Cerebrospinal fluid (CSF) was collected at 1, 2, 3, 4, 5 and 6 h, respectively, and cerebrospinal fluid gold nanoparticles were detected by inductively coupled plasma mass spectrometry. After injecting gold nanoparticles for 6 h, the brain tissue was fixed and fixed, and the ultrastructure of gold nanoparticles and brain tissue were observed under transmission electron microscope. The rat brain tissue was sectioned and stained with Prussian blue, immunohistochemistry and immunohistochemistry Fluorescent staining was used to observe the bleeding and integrity of the blood-brain barrier in rats under light microscope. Results The concentrations of gold nanoparticles in the cerebrospinal fluid of rats gradually increased with time and stabilized after 3 h. Electron microscopy showed that the brain cells of rats were covered with gold nanoparticles and the ultrastructure had some changes. Under light microscope, The blood-brain barrier in rats showed bleeding after Prussian blue staining, and vascular defects were seen after immunohistochemical staining and immunofluorescence staining. Conclusion Nano gold particles can penetrate the blood-brain barrier and cause micro-bleeding and micro-vascular defects.