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采用琼脂块法、拌土育苗和蘸根盆栽生物试验及PPO活性测定法研究供试放线菌对病原菌的皿内拮抗作用、对草莓的促生作用及诱导抗性。结果表明:①供试放线菌对5种草莓土传病害病原菌有明显的抑制作用。Act11对5种病原菌均有较强的抑制作用;Act12对尖孢镰刀菌外的4种病原菌有拮抗作用,Act1对尖孢镰刀菌和草莓疫霉有拮抗作用。②生防菌剂接种促生作用明显,对根系生长的刺激效应尤为显著。在拌土接种育苗处理中,草莓根系及茎叶重量分别较对照提高122.4%~265.6%和53.6%~64.4%。③生防菌接种后可提高草莓新叶和根系PPO活性,产生诱导抗性;根系PPO活性增幅大于叶片。在拌土育苗和蘸根盆栽处理中,草莓新叶PPO活性较对照提高幅度均值分别为5.9%和16.9%;草莓粗根PPO活性提高幅度均值分别为21.4%和35.0%,细根PPO活性提高幅度均值分别为31.3%和26.3%。由此得出结论:供试生防菌在皿内能抑制草莓常见土传病病原菌生长;在拌土育苗和蘸根盆栽条件下均能显著促进草莓根系发育及茎叶生长,并使草莓产生诱导抗性。
The agar block method, mixed soil and dipping root pot experiment and PPO activity assay were used to study the antagonistic effect of actinomycetes on the bacteria in the dish and the promoting effect and inducing resistance on the strawberry. The results showed that: ① The test actinomyces had obvious inhibitory effect on five pathogenic bacteria of soil-borne diseases of strawberry. Act11 has a strong inhibitory effect on the five pathogens; Act12 antagonizes the four pathogenic bacteria outside Fusarium oxysporum, and Act1 has an antagonistic effect on Fusarium oxysporum and Phytophthora capsici. ② biocontrol agent role in promoting growth significantly, the root growth stimulus effect is particularly significant. In the mixed seedling raising seedling treatment, the weight of roots and stems and leaves of strawberry increased by 122.4% ~ 265.6% and 53.6% ~ 64.4% respectively compared with the control. ③ After inoculation, the PPO activity of new leaf and root of strawberry increased, and induced resistance; the PPO activity of root increased more than that of leaf. The results showed that the average PPO activity of strawberries was 5.9% and 16.9% higher than that of the control respectively. The average PPO activity of strawberry roots was increased by 21.4% and 35.0%, and the activity of fine root PPO was increased The mean amplitude was 31.3% and 26.3% respectively. It concludes that the prevention and control bacteria for the test students can inhibit the growth of common pathogen of soil-borne diseases in strawberries. The growth of the root system and the growth of the stems and roots of the strawberry can be significantly promoted under the condition of the soil mixed planting and dipping pots, Induction of resistance.