Room temperature phosphorescence (RTP) generated by small molecules has attracted great attention due to their unique potentials for biosensor, bioimaging and security protection. While, the design of RTP materials is extremely challenging for organic small molecules in non-crystalline solid state. Herein, we report a new strategy for achieving non-crystalline organic small molecules with RTP emission by modifying different phosphors onto diphenylalanine or phenylalanine derivatives. Benefiting from the skeletalstructureof the amino acid derivatives, there are intermolecular hydrogen bond formation and rigidification effect, thereby minimizing the intermolecular motions and enhancing their RTP performance.