魔芋幼芽和幼嫩芽鞘,经0.1%升汞灭菌20分钟后,接种在附加BA.NA(均为0.6m/1)的MS培养基上,在室温25土1℃,每天照光9小时的条件下培养三周,形成愈伤组织。愈伤组织转入附加4(mg/1)BA、0.25(mg/1)IBA,3(mg/1)GA_3的MS分化培养基上,四周后分化出芽和白色地下茎,其分化颖率为40%,再转移至附加2(mg/1)BA、1(mg/1)IBA、0.3%活性炭的MS培养基中,幼茎和叶片从芽鞘中抽出,茎基部分化出根,形成完整的小植株。 Konjac buds and young shoots were sterilized by 0.1% mercuric chloride for 20 minutes and then inoculated on MS medium supplemented with BA.NA (both 0.6m / 1) at 25 ° C and 1 ° C at room temperature for 9 days H for three weeks to form callus. The callus was transferred to MS differentiation medium supplemented with 4 mg / l BA, 0.25 mg / l IBA and 3 mg / l GA_3. After 4 weeks, the shoots and white rhizomes were differentiated with a differentiation rate of 40 %, And then transferred to MS medium supplemented with 2 mg / l BA, 1 mg / l IBA and 0.3% activated charcoal. The young stems and leaves were taken out of the bud sheath and the stem base was partially rooted to form a complete Small plant.