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十几年来藉助提高衬度来改善图象分辨率的暗场成象,在核酸,蛋白质及其它生物材料的精细结构研究中已显示出高分辨率的优越性。本文对核酸暗场成象显微术及其观察结果概述如下: 一、暗场照明我们用JEM—200CX型电镜,采取倾斜照明,取中心暗场象(CDF)的工作模式。由于物镜光阑只接收试样少量信息,因而效率较低。而且光阑一侧受强电子束轰击,易造成光阑的非对称污染,因此必须注意象散的校正。
For more than a decade darkfield imaging has been shown to improve image resolution with increased contrast, which has shown the superiority of high resolution in fine structure studies of nucleic acids, proteins and other biological materials. In this paper, dark field imaging of nucleic acid microscopy and its observations are summarized as follows: First, the dark field lighting We use the JEM-200CX-type electron microscope to take oblique lighting, take the center dark field phenomenon (CDF) mode of operation. The objective aperture is less efficient because it receives only a small amount of sample information. And the diaphragm side of the strong electron beam bombardment, easy to cause the diaphragm asymmetric pollution, it is necessary to pay attention to the correction of astigmatism.