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目的观察不同因素致亚健康模型大鼠血液生化和血气电解质指标的变化。方法 60只雄性Wistar大鼠随机分为5组,即多因素(MF)组、热水游泳(WS)组、睡眠不足(SD)组、单纯束缚(PC)组和正常对照(C)组,每组12只。采用热水游泳、睡眠剥夺和单纯束缚等单因素或多因素联合建立亚健康大鼠模型,各组造模5d后,处死6只,剩余动物进行恢复性饲养3 d后处死。分别测定造模结束后和恢复3 d时大鼠的血清中的丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)、总蛋白(TP)、白蛋白(ALB)、尿素氮(BUN)、肌酐(CREA)、血糖(GLU)、总胆固醇(TC)、甘油三酯(TG)、肌酸磷酸激酶(CK)、乳酸脱氢酶(LDH)和血气电解质等指标。结果 (1)与正常对照(C)组比,造模5d后MF组的大鼠BUN和TG水平显著降低(P<0.05,P<0.01),同时CREA、AST、CK、CK/AST和LDH水平显著升高(P<0.01),WS组的大鼠TC、CK、CK/AST和LDH水平亦升高显著(P<0.01),且各模型组的大鼠GLU浓度明显增加(P<0.01);恢复3 d后,MF组的大鼠TC、AST、ALT、ALB、BUN、CK、CK/AST和LDH水平均明显高于C组(P<0.05,P<0.01);WS组的大鼠血清AST、CK、CK/AST比值和LDH水平亦显著高于C组(P<0.01),SD组的大鼠CK显著高于C组(P<0.05);(2)与C组比,造模5d后MF组、SD组和PC组的大鼠PCO2、Hct和Hb均明显升高(P<0.05,P<0.01),WS组Hct和Hb升高显著且Na+显著降低(P<0.01),MF组的大鼠PO2和SO2%均显著降低且K+明显升高(P<0.05),且各造模组的大鼠HCO3-♂的浓度均显著升高(P<0.05,P<0.01);恢复3 d后,除各造模大鼠的血液pH值和PO2均明显下降(P<0.05,P<0.01)外,MF组、SD组和PC组的大鼠PCO2升高并伴有SO2%的降低(P<0.05,P<0.01),MF组和WS组Na+仍明显低于C组(P<0.05,P<0.01),SD组的大鼠K+和Mg2+则明显高于C组(P<0.05,P<0.01)。结论不同因素诱导的亚健康状态大鼠的血液生化、血气电解质指标均出现了不同程度上的改变,故血液生化和血气电解质指标能有效评估亚健康状态机体血液微环境的改变。
Objective To observe the changes of blood biochemistry and blood gas electrolyte in different sub-health-induced model rats. Methods Sixty male Wistar rats were randomly divided into five groups: the MF group, the WS group, the SD group, the PC group and the C group, 12 in each group. A sub-healthy rat model was established by single factor or multi-factor combination such as hot water swimming, sleep deprivation and simple restraint. After 5 days of modeling, 6 mice were sacrificed and the remaining animals were sacrificed for 3 days and then sacrificed. Serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), total protein (TP), albumin (ALB), blood urea nitrogen (BUN CREA, GLU, TC, TG, CK, LDH and blood gas electrolytes were measured. Results Compared with normal control group (C), the levels of BUN and TG in MF group decreased significantly (P <0.05, P <0.01) and the CREA, AST, CK, CK / AST and LDH (P <0.01). The levels of TC, CK, CK / AST and LDH also increased significantly in WS group (P <0.01), and the concentration of GLU in each model group increased significantly (P <0.01) (P <0.05, P <0.01). The levels of TC, AST, ALT, ALB, BUN, CK, CK / AST and LDH in MF group were significantly higher than those in C group The serum AST, CK, CK / AST ratio and LDH level were also significantly higher in group SD than those in group C (P <0.01). The CK in SD group was significantly higher than that in group C (P <0.05) After 5 days, the PCO2, Hct and Hb in MF, SD and PC groups were significantly increased (P <0.05, P <0.01), and the levels of Hct and Hb in WS increased significantly ), PO2 and SO2% of rats in MF group decreased significantly and K + increased significantly (P <0.05), and the concentration of HCO3-♂ of rats in each model group increased significantly (P <0.05, P <0.01) ). After 3 days of recovery, except for the pH and PO2 in blood of all the model rats decreased significantly (P <0.05, P <0.01), PCO2 in MF group, SD group and PC group increased SO2% (P <0.05, P <0.01), Na + still remained in MF group and WS group Lower than group C (P <0.05, P <0.01), groups of SD rats K + and Mg2 + was significantly higher than group C (P <0.05, P <0.01). Conclusion The blood biochemical and blood gas electrolyte indexes of subhealthy rats induced by different factors have changed to some extent. Therefore, blood biochemical and blood gas electrolyte indexes can effectively evaluate the changes of blood microenvironment in subhealthy rats.