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随着转化医学理念和研究思路的引入,以组学为基础的系统生物学解决复杂问题的优越性得以突显,即通过宏观思路,组装系统方案,解决微观问题,同时通过不同组学在不同研究层面的相互补充,加深了对生命真谛的认识。例如,早期医学研究主要集中在基因水平,由此提出基因组学研究思路,但随着人类基因组计划和全基因组测序完成,科学家们发现很多情况下染色体序列不同,甚至转录水平的变化并不导致表型改变,因而将目光投向基因翻译后的产物蛋白质,并提出蛋白质组学研究方法。磷酸化蛋白质组学是该领域的一个新分支,通过单次试验可以静态评估上千个蛋白的磷酸化情况,发现蛋白新的生理功能,更重要的是可以全局性地研究蛋白磷酸化的动力学变化,观察病理状态下不同蛋白间的相互作用及信号传导的变化,并为寻找药物靶点提供了全新的解决途径。本文将就磷酸化蛋白质组学的特点及其在生命科学中的应用前景作一简述。
With the introduction of translational medicine concepts and research ideas, the superiority of omics-based systems biology in solving complex problems has been highlighted, namely through the macro-thinking and assembly of system solutions to solve micro-problems, and through different omics in different studies Complement each other, deepen the understanding of the true meaning of life. For example, early medical research focused on the genetic level, which led to the idea of genomics research, but with the completion of the human genome project and genome-wide sequencing, scientists have found that chromosomal sequences are different in many cases and even changes in transcription levels do not result in Type of change, which will turn their attention to genetically engineered protein products, and put forward proteomics research methods. Phosphoproteomics, a new branch in the field, enables the static assessment of the phosphorylation of thousands of proteins in a single trial to discover the new physiological functions of proteins and, more importantly, to study the power of protein phosphorylation globally Learn changes, observe the pathological state of the interaction between different proteins and signal changes, and to find a drug target provides a new way to solve. This article will outline the characteristics of phosphoproteomics and its application in life sciences.