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目的:探讨联合羊膜的小片状同种异体结膜移植重建眼表的可行性。方法:新西兰白兔双眼上方球结膜碱烧伤后24h,实验组行以羊膜为基底的小片状同种异体结膜移植,对照组行单纯小片状同种异体结膜移植,术后各周行印迹细胞的PAS染色、扫描电镜检查。结果:实验组未见明显排斥反应,全部植片成活,对照组7眼植片排斥脱落,植片成活率81.6%,明显低于实验组(P<0.05)。实验组术后4wk,对照组术后第5wk结膜外观、杯状细胞形态、数目、功能均恢复正常。结论:羊膜与小片状同种异体结膜移植相结合可能成为修补早期大面积结膜碱烧伤的有效途径。
Objective: To investigate the feasibility of reconstructing ocular surface with conjunctival transplantation of amnion. Methods: New Zealand white rabbits were exposed to bulbar conjunctival conjunctiva transplanted on the base of conjunctiva 24 hours after the conjunctival alkali burns. The control group received simple platelet allograft conjunctival transplantation, PAS staining of cells, scanning electron microscopy. Results: There was no obvious rejection in the experimental group and all the grafts survived. In the control group, the rejection of the grafts in 7 eyes was 81.6%, which was significantly lower than that in the experimental group (P <0.05). The experimental group 4wk after operation, the control group after 5wk conjunctiva appearance, goblet cell morphology, number, function returned to normal. Conclusion: The combination of amniotic membrane and small patchy conjunctival transplantation may be an effective way to repair large area conjunctival alkali burn in early stage.