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应用氮蓝四唑 (NBT)光还原法 ,对六月霜中提取物清除超氧离子自由基O2 ·- 的效果进行了测定 .结果显示 ,六月霜提取物对超氧离子自由基有较强的清除效果 ,清除效果与提取物中黄酮质量浓度有关 ,当黄酮质量浓度达到一定值时 ,对O2 ·- 的清除效果可高达 88.4 1% .提取物与抗坏血酸 (Vc)进行了对照实验 ,结果显示其对O2 ·- 的清除能力比Vc高 .六月霜提取物在Vc—Cu2 +—H2 O2 体系中 ,对OH·自由基的清除效果的研究显示 ,最高清除率为 84 .0 2 % .
The effect of the extracts of June cream on the scavenging of superoxide radical O2 · - was studied by using the NBT photoreduction method. The results showed that the extracts of June cream had better effects on superoxide radical Strong scavenging effect and scavenging effect were related to the concentration of flavonoids in the extract, and when the concentration of flavonoids reached a certain value, the scavenging effect on O2 · - could reach as high as 88.4 1%. The control experiment of extract and ascorbic acid (Vc) The results showed that the scavenging capacity of O2 · - was higher than that of Vc.Studies on the scavenging effect of OH · Radicals in the Vc-Cu2 + -H2 O2 system showed that the highest clearance rate was 84.02 %.