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目的:研究PBK/TOPK在TPA(佛波酯)诱导白血病细胞HL-60的分化时的表达及意义.方法:细胞化学染色法观察药物作用后细胞形态的变化;流式细胞仪检测药物对HL-60细胞周期及细胞表面分化抗原CD11b,CD14,CD13和CD33表达的影响;用westernBlot法检测PBK/TOPK在HL-60细胞分化前后表达的变化.结果:经5.1nmol/LTPA处理72h后,NBT阳性细胞数和CD11b,CD13,CD14表达是增加的,HL-60细胞体积缩小,核浆比例减低,核仁减少甚至消失,核形态扭曲折迭或分叶;PBK/TOPK在HL-60细胞向成熟分化后表达下调.结论:TPA能抑制白血病细胞HL-60的增殖,并诱导其向成熟单核、粒细胞分化,在此过程中,PBK/TOPK表达是下调的,但Pho-PBK/TOPK的表达是增加的.
OBJECTIVE: To study the expression and significance of PBK / TOPK in the differentiation of HL-60 cells induced by TPA (phorbol ester) .Methods: Cytochemical staining was used to observe the morphological changes of cells after the drug was given. Flow cytometry -60 and the expression of cell surface differentiation antigens CD11b, CD14, CD13 and CD33 were detected by western blotting.Results: After treatment with 5.1nmol / L TPA for 72h, NBT The number of positive cells and the expression of CD11b, CD13 and CD14 were increased. The volume of HL-60 cells was reduced, the proportion of cytoplasm was reduced, nucleoli were reduced or even disappeared, Down-regulated after maturation differentiation.Conclusion: TPA can inhibit the proliferation of HL-60 leukemia cells and induce its differentiation into mature monocytes and granulocytes. In this process, PBK / TOPK expression is down-regulated, but Pho-PBK / TOPK The expression is increased.