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目的：在体外观察８肽生长抑素（ＳＭＳ）对原代培养大肠癌活细胞数和ＤＮＡ合成的影响，以探讨生长抑素对大肠癌细胞生长的调控作用。方法：对２５例大肠癌根治术病人的新鲜标本进行癌细胞分离和原代培养，采用ＭＴＴ比色分析法检测活癌细胞数（吸光度，Ａ值）；３Ｈ－ＴｄＲ掺入法检测ＤＮＡ合成（脉冲数，ＣＰＭ值）。分ＳＭＳ组、５肽胃泌素（ＰＧ）组、ＳＭＳ＋ＰＧ组及对照组。结果：ＳＭＳ组高、中和低分化腺癌活癌细胞数和ＤＮＡ合成均明显低于对照组（Ｐ均＜０．０５）和ＰＧ组（Ｐ均＜０．０１），ＰＧ组均显著高于对照组（Ｐ均＜０．０１），ＳＭＳ＋ＰＧ组均显著低于对照组（Ｐ＜０．０１～０．０５）及ＰＧ组（Ｐ均＜０．０１）。结论：生长抑素对原代培养大肠癌细胞的生长具有负性调控作用，它不仅具有直接抑制大肠癌细胞生长的作用，而且具有抑制胃泌素对大肠癌细胞的促生长作用，为大肠癌病人应用生长抑素类似物治疗提供实验依据。
OBJECTIVE: To observe the effect of 8-peptide somatostatin (SMS) on the number of viable cells and DNA synthesis of primary cultured colorectal cancer in vitro, and to explore the regulation of somatostatin on the growth of colorectal cancer cells. METHODS: Cancer cells were isolated and cultured in 25 specimens of patients undergoing radical colorectal cancer surgery. The number of viable cancer cells (absorbance, A value) was measured by MTT colorimetric assay; DNA synthesis was detected by 3H-TdR incorporation method ( Number of pulses, CPM value). The patients were divided into SMS group, 5-gastrin gastrin (PG) group, SMS+PG group and control group. RESULTS: The number of viable cancer cells and DNA synthesis in the high, middle and poorly differentiated adenocarcinomas in the SMS group were significantly lower than those in the control group (P <0.05) and the PG group (P <0.01). The PG group was significantly higher. In the control group (P <0.01), SMS + PG group were significantly lower than the control group (P <0.01 ~ 0.05) and PG group (P <0.01). Conclusion: Somatostatin has a negative effect on the growth of primary cultured colorectal cancer cells. It not only has the function of directly inhibiting the growth of colorectal cancer cells, but also has the effect of inhibiting the growth promoting effect of gastrin on colorectal cancer cells. Patients provided experimental evidence for the use of somatostatin analogue therapy.