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目的探讨尘肺患者血中一氧化氮(NO)、丙二醛(MDA)和超氧化物歧化酶(SOD)的浓度变化在尘肺发病中的作用和意义。方法选择某企业100名尘肺患者和105健康工人作为调查对象,采用硝酸还原酶法、硫代巴比妥酸显色法、黄嘌呤氧化酶法测定血清中NO、MDA的含量及SOD的活力。结果尘肺患者血清中NO和MDA含量高于对照组(P<0.05),SOD活力低于对照组(P<0.05),并且随着尘肺期数的增高,血清中NO的含量呈增加的趋势(P<0.05),而MDA浓度变化SOD活力变化不明显(P>0.05)。尘肺患者血清中NO含量与SOD的活力之间呈负相关关系(P<0.05),而NO含量与MDA及MDA与SOD之间无相关关系(P>0.05)。对照组人群血清中3项指标之间均无相关关系(P>0.05)。结论尘肺患者体内氧自由基反应异常,氧化/抗氧化状态严重失衡。
Objective To investigate the role and significance of changes of blood levels of nitric oxide (NO), malondialdehyde (MDA) and superoxide dismutase (SOD) in the pathogenesis of pneumoconiosis. Methods 100 pneumoconiosis patients and 105 healthy workers in a certain enterprise were selected as research objects. The content of NO and MDA and the activity of SOD in serum were determined by nitrate reductase method, thiobarbituric acid colorimetric method and xanthine oxidase method. Results The serum levels of NO and MDA in patients with pneumoconiosis were significantly higher than those in the control group (P <0.05), while the activities of SOD in the pneumoconiosis patients were lower than those in the control group (P <0.05) P <0.05), but there was no obvious change in MDA concentration (P> 0.05). There was a negative correlation between the content of NO in serum of pneumoconiosis patients and the activity of SOD (P <0.05), while there was no correlation between the content of NO and MDA, MDA and SOD (P> 0.05). There was no correlation between the three indexes in the control group (P> 0.05). Conclusion The patients with pneumoconiosis have abnormal oxygen free radical reaction and a serious imbalance of oxidation / anti-oxidation status.