This paper aims at synthesizing a detergent with graphene oxide ( GO) and lauryl betaine ( LB) , where the GO will have a better performance with the modification of LB. First, the complexus of GO and LB and the complexus of GO, KH550 ( Amino functional silane) , and LB were synthesized to obtain two products, GO-LB and GO-KH550-LB. Then, the abilities and safety of the products were evaluated by characterization, contact angle measurement, measurement of KRAFFT Point, foaming test, emulsion ability test, decontamination capacity test, skin irritation test, and skin allergy test. Results of the experiment showed that GO-LB was superior to GO-KH550-LB in wetting ability and foaming stability, while GO-KH550-LB outperformed in emulsifying capacity. Besides, good abilities of removing mussels, seaweed, and protein were proved. According to the result of the irritation test of the materials to skin, they were both at the light irritation level, and the irritation of the GO-LB was slightly lower than that of GO-KH550-LB. Neither GO-LB nor GO-KH550-LB had allergic effect. Therefore, it can be concluded that as a surface active agent, graphene oxide/lauryl betaine was effective as a new detergent, which had low irritation and insignificant allergic phenomena on the human body over a long term of use.