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目的:探讨可口革囊星虫多肽在SD大鼠运动模型中的抗氧化功效。方法:用传统水煮方法提取星虫蛋白质,同时用蛋白酶将提取的蛋白质水解成小肽,并用阳离子交换层析柱对星虫肽进行纯化,最后采用冷冻干燥制备出星虫肽。50只雄性SD大鼠分为运动组和安静组两大组,每组又分为对应的4小组:分别将配制好的高、低剂量的星虫肽溶液灌胃补充给相对应组大鼠,同时设计了生理盐水、星虫蛋白组作为对照;运动组进行游泳训练;2周后各组大鼠取材测试,用血细胞分析仪测定血液RBC、Hb与HCT等指标,采用生化试剂盒测定血清SOD、GSH-PX、NOS活性,肝组织SOD、GSH-PX活性,肝组织LPO和腓肠肌糖原含量。结果:(1)星虫蛋白质的分子量主要由66kd以上、27kd、9.5kd和4.1～6.5kd四部分组成,水解度后星虫肽分子量主要分布在1.0～4.1kd之间。(2)低剂量星虫肽运动组大鼠RBC、Hb、HCT水平(6.80×1012/L、133.7g/L、35.3%)与生理盐水运动组和星虫蛋白运动组比较显著提高(P<0.05)。高、低剂量星虫肽运动组血清与肝组织的SOD、GSH-PX活性相对生理盐水运动组和星虫蛋白运动组均明显提高(P<0.05),且高剂量组比低剂量组提高更显著;高、低剂量运动组之间血清SOD、SH-PX活性有显著差异(P<0.05),高、低剂量运动组之间的肝组织GSH-PX活性有显著性差异(P<0.05);高、低剂量星虫肽运动组血清NOS和肝组织LPO含量显著低于生理盐水运动组和星虫蛋白运动组(P<0.05)。高、低剂量星虫肽运动组腓肠肌糖原含量低于生理盐水运动组和星虫蛋白运动组,而高、低剂量星虫肽安静组腓肠肌糖原含量高于生理盐水和星虫蛋白安静组,但无显著性差异(P>0.05)。结论:可口革囊星虫多肽可提高大鼠RBC、Hb、HCT水平,同时提高血清SOD、GSH-PX活性,降低血清NOS活性,降低肝组织LPO含量,因此星虫肽有抗组织缺血、抗氧化等功效,是一种有效的运动抗氧化剂。
OBJECTIVE: To investigate the anti-oxidative effect of the polysaccharide from S. caspase in SD rat model. Methods: The traditional method of boiling water was used to extract the star-worm protein. At the same time, the extracted protein was hydrolyzed into small peptides by protease, and the satellite was purified by cation exchange chromatography. Finally, the freeze-dried starches peptide was prepared. Fifty male Sprague-Dawley rats were divided into two groups: exercise group and quiet group, and each group was divided into four groups: the high and low dose of prepared astrocytoma solution were intragastrically fed to corresponding rats At the same time, physiological saline and echinoderm were designed as control; exercise group was trained in swimming; after 2 weeks, the rats in each group were drawn and tested. Blood RBC, Hb and HCT were determined by hematology analyzer. SOD, GSH-PX, NOS activity, liver SOD, GSH-PX activity, liver tissue LPO and gastrocnemius muscle glycogen content. Results: (1) The molecular weight of Astrovirus was mainly composed of more than 66kd, 27kd, 9.5kd and 4.1 ~ 6.5kd. The molecular weight of the latter was mainly distributed between 1.0-4.1kd. (2) The levels of RBC, Hb and HCT (6.80 × 1012 / L, 133.7g / L, 35.3%) were significantly increased in the low dose eschoteric exercise group compared with the saline exercise group and the nematode protein exercise group (P < 0.05). The activity of SOD and GSH-PX in serum and liver of high and low dose eschin-peptide exercise group were significantly higher than that of saline exercise group and the nematode protein exercise group (P <0.05), and the high-dose and low- (P <0.05). The activities of GSH-PX in liver tissue of high and low-dose exercise groups were significantly different (P <0.05) Serum NOS and hepatic LPO content of high and low dose of astrocytoma exercise group was significantly lower than that of saline exercise group and the control group (P <0.05). The gastrocnemius muscle glycogen content of the high and low dose eschotermin exercise groups was lower than that of the saline exercise group and the stellate protein exercise group, while the gastrocnemius muscle glycogen content of the high and low dose esophageal peptide resting groups was higher than that of the saline and the nematode protein quiet group , But no significant difference (P> 0.05). CONCLUSION: The S. cerealis polypeptide can increase RBC, Hb and HCT levels, increase serum SOD, GSH-PX activity, decrease serum NOS activity and decrease LPO content in liver tissue. Therefore, Antioxidant and other effects, is an effective exercise antioxidant.