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从杀虫植物杠柳Periploca sepium Bunge根皮中分离得到的杠柳新苷P具有很高的杀虫活性,为了探索其杀虫机理,采用经典的昆虫蛋白酶活性测定方法,比较研究了杠柳新苷P和无杀虫活性的杠柳新苷E对东方粘虫Mythimna separata与小地老虎Agrotis ypsilon6龄幼虫中肠类胰蛋白酶和类胰凝乳蛋白酶活性的影响。结果表明:对东方粘虫弱碱性类胰蛋白酶,杠柳新苷P表现出强激活作用(酶活性为对照的3.43倍),激活时间可长达8h,而杠柳新苷E则无明显激活作用。杠柳新苷P和E对东方粘虫弱碱性类胰蛋白酶活性的影响二者差异显著(P=0.01),杠柳新苷P药后2,4和8h,东方粘虫中肠弱碱性类胰蛋白酶的活性分别是杠柳新苷E药后的15.4,106.8和242.7倍。酶活性测定结果还表明,与东方粘虫相比,小地老虎中肠类胰蛋白酶活性相对较低,且杠柳新苷P的激活作用也较弱,这可能是杠柳新苷P对东方粘虫具杀虫活性,而小地老虎对其不敏感的原因之一;另外,杠柳新苷P和E对试虫中肠类凝乳胰蛋白酶活性均无明显影响。据此推测,杠柳活性成分新苷P对东方粘虫中肠弱碱性类胰蛋白酶的激活作用可能是造成试虫中毒的机理之一。
Insecticidal plant Periploca sepium bunge bark Phylloside P isolated from the root bark has a high insecticidal activity, in order to explore its insecticidal mechanism, the use of classic insect protease activity assay, a comparative study of bar Liu new Glucosidase P and no pesticidal activity of biluixin glycosides on the trypsin and chymotrypsin activities in midguts of Mythimna separata and Agrotis ypsilon sixth instar larvae of Mythimna separatea. The results showed that plectliosin P showed potent activity (3.43 folds of the control) against Mythimna separata, a weakly basic tryptase, and its activation time could be as long as 8 h, while glucagonogin E showed no obvious effect Activation. Psalidixins P and E on the southern armyworm weak alkaline tryptase activity between the two significant differences (P = 0.01), Psarizedoside P drug 2, 4 and 8h, the Eastern armyworm midgut weak base The activity of tryptase is 15.4, 106.8 and 242.7 times that of glufosinate E respectively. The results of enzyme activity assay also showed that, compared with the oriental armyworm, midgut trypsin activity is relatively low, and the action of glucosinolate P is weak, which may be glucosinolates P oriental The insecticidal activity of Mythimna separata was one of the reasons insects were not sensitive to it. In addition, Psalidone P and E had no significant effect on intestinal curd trypsin activity in the test insects. It is speculated that the active ingredient of pilosus P on the oriental armyworm midgut weak alkaline tryptase activation may be one of the mechanisms leading to test insects poisoning.