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目的探讨红细胞(RBCs)储存损伤对大肠杆菌感染的免疫力低下大鼠输血后死亡率的影响。方法将69只SPF级SD大鼠,根据是否使用免疫抑制剂(环磷酰胺和地塞米松)和输注RBCs的储存时间不同,随机分成3个实验组:免疫抑制+9 d RBCs组、免疫抑制+1 d RBCs组和免疫抑制+生理盐水(NS)组(18只/组);1个正常对照组:单纯NS组,含15只大鼠。每只大鼠经股静脉放出全身20%血液后,分别输注与失血量相同的储存9或1 d的浓缩RBCs及等量NS,随后植入大肠杆菌溶液0.5 mL,并连续观察7 d。术后24 h,取尾静脉血浆用亚铁嗪微板法测定血清铁,并以LB培养基血细菌培养15 h,用紫外分光光度仪测定OD600;术后24 h处死各免疫抑制组大鼠3只/组,取组织做病理检查。结果 3个免疫抑制组和对照组分别输注9、1 d RBCs和NS后的大鼠死亡率分别为10/15,11/15和9/15(P>0.05),死亡大鼠的平均生存时间(h)分别为55.6±21.3,38±16.6和28.3±15.0(P<0.05);24 h血清铁离子浓度(μmol/L)分别为34.1±9.8,28±10.2,26.8±9.0和22.4±5.9(P>0.05);血细菌培养的结果(OD_(600))分别为0.510±0.340,0.790±0.281,0.427±0.107和0.349±0.069(P<0.05);24 h回肠组织细菌计数(个/1 000倍视野)分别为44±9,101±7和27±8(P<0.05)。结论输注不同储存时间的RBCs对于大肠杆菌感染的免疫力低下大鼠的死亡率没有明显影响;但是相比储存时间长的RBCs,新鲜RBCs输注后明显促进了免疫力低下大鼠体内大肠杆菌的增值并加速了感染大鼠的死亡。
Objective To investigate the effect of storage injury of erythrocyte (RBCs) on post-transfusion mortality in E. coli infected mice with low immunity. Methods Sixty-nine Sprague-Dawley (SD) SD rats were randomly divided into 3 experimental groups: immunosuppressed + 9 d RBCs group, immunized according to whether the immunosuppressants (cyclophosphamide and dexamethasone) 1 d RBCs group and immunosuppressive + NS group (18 rats / group). One normal control group: NS group only, containing 15 rats. After releasing 20% of the whole body blood via the femoral vein, rats were respectively transfused with concentrated RBCs of 9 or 1 day and an equal volume of NS with the same amount of blood loss, followed by implantation of 0.5 mL of E. coli solution for 7 days. At 24 h after operation, serum iron was obtained from the tail vein by ferrous oxalate microplate assay and cultured in LB medium for 15 h. OD600 was measured by UV spectrophotometer. All immunosuppressive rats were sacrificed 24 hours after operation 3 / group, take tissue for pathological examination. Results The mortality rates of rats in the three immunosuppressive groups and the control group after 9 and 1 d RBCs and NS infusions were 10/15, 11/15 and 9/15, respectively (P> 0.05). The mean survival of the dead rats (H) were 55.6 ± 21.3,38 ± 16.6 and 28.3 ± 15.0 respectively (P <0.05). The 24 h serum iron concentration (μmol / L) were 34.1 ± 9.8,28 ± 10.2,26.8 ± 9.0 and 22.4 ± 5.9 (P> 0.05). The results of blood culture (OD 600) were 0.510 ± 0.340,0.790 ± 0.281,0.427 ± 0.107 and 0.349 ± 0.069 respectively (P <0.05) 1 000-fold field of view) were 44 ± 9, 101 ± 7 and 27 ± 8, respectively (P <0.05). CONCLUSION: RBCs with different storage time have no significant effect on the mortality of E. coli infected mice with low immunity. However, compared with the RBCs with long storage time, fresh RBCs infusion significantly promoted the growth of E. coli Added value and accelerated the death of infected rats.