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利用植原体16S rRNA基因及核糖体蛋白基因(ribosomal protein,rp)通用引物对发生在云南元谋的花生丛枝病病株DNA进行PCR扩增,并对扩增片段进行序列测定。扩增获得的云南元谋花生丛枝植原体(PnWB-YNym)16S rDNA、16S-23S rDNA和23S DNA片段总长1 806 bp,rp基因扩增片段长1 171 bp。云南株系与来源于台湾和海南的花生丛枝植原体均有较高同源性。比较16S rDNA片段,发现云南株系在5个位点上与来自台湾或海南的株系存在碱基差异,其中有1个位点的差异是云南元谋株系特异的;再分别比较核糖体蛋白rplV-rpsC 2个基因所编码的氨基酸序列,发现云南株系rpsC编码的第194位氨基酸与台湾和海南的株系存在差异。经16S rDNA片段系统进化及iPhyClassifier在线分析,表明PnWBYNym在分类上属于16SrII-A亚组成员,与候选种‘Cand/datus Phytoplasma australasiae’相关;基于rp基因构建的系统进化树表明,PnWB-YNym与16SrII-A亚组各成员聚为同一亚进化支(iii)。
The genomic DNAs of peanut broussoneurosis in Yuanmou, Yunnan were amplified by PCR using 16S rRNA gene and ribosomal protein (rp) universal primers. The amplified fragments were sequenced. The total length of 16S rDNA, 16S-23S rDNA and 23S DNA fragments of PnWB-YNym amplified in Yuanmou, Yunnan was 1 806 bp in length and 1 171 bp in amplified fragment of rp gene. Yunnan strains have a higher homology with peanut taxa from Taiwan and Hainan. Comparison of 16S rDNA fragments showed that there were base differences between Yunnan lines and those from Taiwan or Hainan at one of the five loci. One locus was found to be specific to the Yuanmou Yunnan lineage. Ribosomal protein rplV-rpsC. The results showed that the amino acid sequence of amino acid at position 194 encoded by rpsC in Yunnan was different from those in Taiwan and Hainan. Phytoplasma australasiae (Candidate) and PnWB-YNym (Phytoplasma australasiae). The phylogenetic tree based on the rp gene showed that PnWB-YNym and Members of the 16SrII-A subgroup clustered together into the same sub-clade (iii).