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观察力达霉素(LDM)、LDM与甲氨蝶呤(MTX)联合用药对人纤维肉瘤HT-1080LUC实验性肺转移的抑制作用。构建稳定表达荧光素酶的HT-1080细胞株并扩增培养,检测HT-1080LUC细胞的荧光素酶的表达。建立裸鼠人纤维肉瘤HT-1080LUC实验性肺转移模型,并采用活体动物成像系统监测肿瘤的生长情况,观察LDM和MTX的体内抗肺转移瘤活性。结果表明,HT-1080LUC细胞荧光光子量与细胞数呈线性相关,最小可检测的细胞数量为100个/孔。活体成像结果显示,治疗组裸鼠的肺部荧光强度较对照组明显减弱。单独给予LDM 0.025 mg.kg-1、LDM 0.05 mg.kg-1或MTX 0.5 mg.kg-1的肺转移抑制率分别为53.9%、75.9%和70.2%;LDM 0.025 mg.kg-1与MTX 0.5 mg.kg-1联合应用的肺转移抑制率为88.7%,两药相互作用指数CDI=0.82。研究表明,LDM对人纤维肉瘤肺转移有明显抑制作用,与MTX联合用药对肺转移瘤的疗效明显优于单独给药。
To observe the inhibitory effect of combination of LDM, LDM and methotrexate (MTX) on the experimental lung metastasis of human fibrosarcoma HT-1080LUC. The HT-1080 cell line stably expressing luciferase was constructed and expanded and cultured to detect the luciferase expression in HT-1080LUC cells. The experimental lung metastasis model of human fibrosarcoma HT-1080LUC was established. The growth of tumor was monitored by live animal imaging system and the in vivo anti-lung metastatic activity of LDM and MTX was observed. The results showed that the fluorescence photon quantity of HT-1080LUC cells was linearly correlated with the number of cells, the minimum detectable cell number was 100 cells / well. The results of in vivo imaging showed that the lung fluorescence intensity of nude mice in treatment group was significantly weaker than that in control group. The inhibition rates of lung metastases of LDM 0.025 mg.kg-1 alone, LDM 0.05 mg.kg-1 or MTX 0.5 mg.kg-1 were 53.9%, 75.9% and 70.2%, respectively. LDM 0.025 mg.kg-1 and MTX 0.5 mg.kg-1 combined application of lung metastasis inhibition rate was 88.7%, the two drug interaction index CDI = 0.82. Studies have shown that, LDM on human fibrosarcoma lung metastasis was significantly inhibited, combined with MTX lung metastases efficacy was significantly better than single administration.