Aim: The aim of this study is to evaluate the efficacy of the protocol of nanofat and platelet-rich plasma (PRP) infiltration and fractional CO2 laser resurfacing for face atrophic scars by acne and to analyse patient’s satisfaction with the original questionnaire.Methods: From March 2014 to June 2015, 30 patients with acne scarsonthe cheeks were selected for this pilot study. Patients were evaluated pre- and postoperatively by a physical examination, photographs and ultrasound scan with a 22 MHz probe to measure subcutaneous tissue thickness. All patients were treated by infiltration of nanofat and PRP. The production of PRP was achieved by RegenLab THT tube?method. Subsequently, patients were randomly divided into two groups: group A, which also underwent a fractional CO2 laser resurfacing at 15 W; and group B, which only underwent nanofat and PRP infiltration. The original questionnaire of quality of life questionnaire (QoL-Q) was also administered pre- and postoperatively to analyse satisfaction and aesthetical perception of the result.Results: Preoperative thickness of subcutaneous tissue of patients from group A was 0.532 mm, whilst preoperative thickness of subcutaneous tissue of patients from group B was 0.737 mm. Postoperative thickness of subcutaneous tissue was 1.201 mm in group A and 1.367 mm in group B. The improvement of thickness of subcutaneous tissue was 0.668 mm in group A and 0.630 mm in group B. The authors applied a t-test on unpaired data, comparing the difference in thickness obtained with the treatment both in group A and in group B, with aP = 0.7289 (not significant). All patients in both groups had a treatment benefit.Conclusion:Combined approach with nanofat, PRP and CO2 laser seems to be effective to improve trophic scars, however, infiltration alone proved to significantly increase skin and subcutaneous tissue thickness. QoL-Q confirmed the impact of acne scars on the face in social life and relationships.