论文部分内容阅读
骨髓增生异常综合征(MDS)是近几年来开始研究的一组疾病。难治性贫血是MDS的最初阶段,其关键是病态造血,以贫血为主要症状,用VitB_(12)和叶酸等治疗效果不佳,有一部分病例发展为急性白血病。方法一、病例选择我们所选的14例难治性贫血病例均符合1982年FAB协作组有关MDS-RA的分型诊断标准。男8例。女6例,平均年龄37·14岁,病程1—3年均未用过激素和进行化疗。以健康献血员作为对照。二、植物血凝素(PHA)刺激剂浓度试验无菌抽取正常人静脉血15ml,加无菌肝索钠抗凝(50—60u/ml),加等量无血清RPMI 1640(U.S.A产品)培养液混匀后,用淋巴细胞分层液(上海试制二厂产品)进行分离,以2000rpm速度离心20分
Myelodysplastic syndrome (MDS) is a group of diseases that have been studied in recent years. Refractory anemia is the initial stage of MDS, the key is morbid hematopoiesis, with anemia as the main symptom, with VitB_ (12) and folic acid and other poor treatment, in some cases the development of acute leukemia. Method 1, case selection 14 cases of refractory anemia we selected were in line with the 1982 FAB collaboration group MDS-RA classification of diagnostic criteria. Male 8 cases. 6 females, the average age of 37.14 years of age, duration of 1-3 years have not used hormones and chemotherapy. Take healthy blood donors as a control. Phosphatidylcholine (PHA) stimulant concentration test Normal human venous blood 15ml sterile extraction of aseptic hepatic sodium anticoagulant (50-60u / ml), plus the same amount of serum-free RPMI 1640 (USA product) culture After the solution was mixed, the cells were separated by a lymphocyte stratified solution (Shanghai Trial Plant No. 2) and centrifuged at 2000 rpm for 20 minutes