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目的研究各型乙型病毒性肝炎患者外周血T淋巴细胞亚群的变化,以及在病毒复制状态下免疫功能的紊乱情况。方法应用流式细胞仪检测T淋巴细胞亚群,荧光定量PCR法测定HBV-DNA。结果慢性乙型病毒性肝炎患者CD4+/CD8+值较正常对照组下降。肝硬化组与正常组无显著性差异。在乙型肝炎病毒复制组与不复制组CD4+/CD8+有显著性差异。结论乙型病毒性肝炎为免疫损伤所致,在慢性肝炎及乙型肝炎病毒复制状态下存在严重免疫功能紊乱。
Objective To study the changes of T lymphocyte subsets in peripheral blood of patients with various types of hepatitis B virus and the disturbance of immune function in the state of virus replication. Methods T-lymphocyte subsets were detected by flow cytometry and HBV-DNA by fluorescence quantitative PCR. Results The CD4 + / CD8 + of patients with chronic hepatitis B decreased compared with the control group. Liver cirrhosis group and normal group no significant difference. There was a significant difference in CD4 + / CD8 + between hepatitis B virus replication group and non-replication group. Conclusion Hepatitis B virus is caused by immune injury, and there is a serious immune dysfunction in chronic hepatitis and hepatitis B virus replication.