目的:研究全民健身背景下有氧运动对持续性有机污染物代谢的影响,揭示有氧运动对DDT在体内累积代谢、对肝脏应激及结构的影响。方法:90只雄性Wistar大鼠随机分为对照组(CON)、安慰剂组(PG)、有氧运动组(AG)、正常饲养DDT组(CDG)、有氧运动DDT组(ADG)。其中,CDG和ADG以40mg/kg(体重)的比例喂食DDT溶液2周,ADG和AG每天以18m/min运动30min。在运动第15天、30天、45天后取材,经研磨和冷冻干燥后采用气相色谱法测定不同组织中DDT的浓度分布;采用黄嘌呤氧化酶法测定肝脏SOD活性、硫代巴比妥酸比色法测定肝脏MDA含量、酶速率法测试肝脏GSH-Px活性、可见光法测定肝脏CAT活性;HE染色肝脏组织切片、醋酸铀和柠檬酸铅对超微结构染色,分别采用DP70数码显微镜成像系统及Tecnai Spirit 120KV透射电子显微镜及成像系统观察、拍照。结果:CDG肝脏中DDT浓度在停止喂食DDT后呈逐渐下降趋势,而ADG中则出现浓度先上升后下降的趋势。在15~30天中,ADG肝脏中DDT浓度显著高于CDG(P<0.01),而45天则显著低于CDG(P<0.05);与CDG比较,ADG心脏中DDT浓度在15天和45天时显著升高,而30天时DDT浓度低于CDG组;CDG骨骼肌DDT浓度呈递减趋势,ADG中呈逐渐上升趋势;在15~30天,ADG血液DDT浓度显著低于CDG(P<0.01);CDG中粪便DDT浓度呈逐渐下降趋势,ADG中浓度呈现较为稳定的趋势。氧化应激研究发现,有氧运动促进ADG中SOD活性在15~45天持续升高,且显著性高于CDG(P<0.05);MDA含量在15~30天内显著性升高(P<0.05),但在45天时呈现显著性下降(P<0.01);GSH-Px及CAT活性15天和30天时均显著升高(P<0.05),在45天时则受到显著抑制(P<0.05)。DDT摄入影响肝脏超微结构,并随着DDT在肝脏中积累而加重,施加有氧运动干预后期能明显改善DDT摄入引起的超微结构的变化。结论:中等强度运动通过改变DDT在体内分布,减少了DDT在肝脏中的富集,但增加了运动初期心脏和骨骼肌中DDT分布及DDT排出体外的速度;改善肝脏的氧化应激状态,特别有利于运动初期肝脏中氧化应激状态的改善;有利于DDT富集引起的肝组织结构变化恢复。 Objective: To study the effect of aerobic exercise on persistent organic pollutants metabolism under the background of national fitness and to reveal the effect of aerobic exercise on cumulative metabolism of DDT in vivo and on liver stress and structure. Methods: 90 male Wistar rats were randomly divided into control group (CON), placebo group (PG), aerobic exercise group (AG), normal diet DDT group (CDG) and aerobic exercise DDT group (ADG) Among them, CDG and ADG were fed DDT solution at a rate of 40 mg / kg (body weight) for 2 weeks, ADG and AG were exercised at 18 m / min for 30 min every day. After 15 days, 30 days and 45 days of exercise, the concentration of DDT in different tissues was determined by gas chromatography after grinding and freeze-drying. The activity of SOD and the activity of thiobarbituric acid in liver were measured by xanthine oxidase method The activity of GSH-Px in the liver was measured by colorimetric method, and the activity of GSH-Px in liver was measured by enzymatic rate method. CAT activity of liver was measured by visible light method. The liver tissue sections were stained with HE, lead acetate and lead citrate were stained with DP70 digital microscope system Tecnai Spirit 120KV transmission electron microscopy and imaging system observation, taking pictures. Results: DDT concentration in CDG liver decreased gradually after DDT was stopped, while ADG increased first and then decreased. DDT concentration in ADG liver was significantly higher than that in CDG (P <0.01) and significantly lower than that in CDG at 45 days (P <0.05). Compared with CDG, the concentration of DDT in ADG heart was lower at 15 and 45 days The DDT concentration in CDG skeletal muscle showed a decreasing trend and the level in ADG showed a gradual upward trend; in 15-30 days, DDT concentration in ADG blood was significantly lower than that in CDG group (P <0.01) The DDT concentration of feces in CDG showed a gradual downward trend while the concentration of ADG showed a relatively stable trend. Oxidative stress study found that aerobic exercise to promote SOD activity in ADG continued to increase 15 to 45 days, and significantly higher than CDG (P <0.05); MDA content increased significantly in 15 to 30 days (P <0.05 ), But decreased significantly at 45 days (P <0.01). GSH-Px and CAT activity were significantly increased at 15 days and 30 days (P <0.05) and significantly inhibited at 45 days (P <0.05). DDT intake affects the ultrastructure of the liver, and as DDT accumulates in the liver and aggravate, aerobic exercise can significantly improve the post-interventional changes in ultrastructure caused by DDT uptake. Conclusion: Moderate-intensity exercise can reduce DDT accumulation in the liver by changing the distribution of DDT in the body, but increase the distribution of DDT in the heart and skeletal muscle and the speed of excretion of DDT in the heart; improve the oxidative stress in the liver, especially Which is conducive to the improvement of oxidative stress in the liver in the early stage of exercise. It is also conducive to the restoration of liver tissue structure changes caused by enrichment of DDT.