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目的 探讨纳米细菌在正常人群血清及胆囊结石患者血清、胆汁和胆囊黏膜中的分布情况。 方法 ( 1)采用ELASA方法测定 338例正常人群和 76例胆囊结石患者血清中纳米细菌的感染情况 ;( 2 )对 5 7例胆囊结石和 18例非胆囊结石患者的胆囊胆汁进行纳米细菌培养 ,并采用免疫组化染色和透射电镜扫描进行鉴定 ;( 3)随机选取其中 40例胆囊结石患者胆囊黏膜进行免疫组化染色 ,观察相应胆汁和胆囊黏膜中纳米细菌分布的差异。 结果 ( 1)正常人群和胆囊结石患者血清中纳米细菌感染率分别为 8 0 %和 31 6 % ,两者间差异有显著性 ( χ2 =2 9 90 ,P <0 0 5 ) ;( 2 ) 75例胆汁中有46例感染纳米细菌 ,阳性率为 6 1 3 % ,其中胆囊结石患者的胆汁纳米细菌的感染率为 6 1 4 % ,非胆囊结石患者的胆汁纳米细菌的感染率为 6 1 1% ,两者间并无统计学差异 ( χ2 =0 89,P >0 0 5 )。 ( 3) 14例胆囊结石患者胆囊黏膜感染纳米细菌 ,阳性率为 35 0 % ,其主要分布在黏膜或黏膜下层及钙化灶。 结论 正常人群血清中纳米细菌感染率为 8 0 % ,胆囊结石患者血清、胆汁和胆囊黏膜中存在着纳米细菌感染 ;胆囊壁的钙化或纤维化可能与纳米细菌感染有关。
Objective To investigate the distribution of nano-bacteria in the serum, bile and gallbladder mucosa in patients with normal serum and gallstone. Methods (1) ELASA method was used to detect the infection of nBacteria in the serum of 338 normal people and 76 patients with gallstone disease. (2) Nanobacteria were cultured in gallbladder bile of 57 patients with gallstones and 18 patients with non-gallbladder stones, And immunohistochemical staining and transmission electron microscopy were used for identification. (3) 40 cases of cholecystolithiasis were randomly selected for immunohistochemical staining of gallbladder mucosa to observe the difference of the distribution of nodule bacteria in the corresponding bile and gallbladder mucosa. Results (1) The nosocomial infection rates of normal and gallbladder patients were 80% and 31 6%, respectively, with significant difference (χ2 = 29 990, P <0 05). (2) Among 75 cases of bile, 46 cases were infected with Nano-bacteria, the positive rate was 61.3%. The infection rate of bile nano-bacteria was 61.4% in patients with gallstone disease and 61% in non-gallstone patients 1%, there was no significant difference between the two (χ2 = 0 89, P> 0 0 5). (3) Among the 14 patients with gallbladder stones, gallbladder mucosa was infected with nano-bacteria, the positive rate was 35%, which mainly distributed in the mucosa, submucosa and calcification. Conclusion The prevalence of nosocomial infection in normal population is 80%. There is nosocomial infection in serum, bile and gallbladder mucosa in gallbladder patients. Calcification or fibrosis of gallbladder wall may be related to nosocomial infection.