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通过对红螯螯虾(Cherax quadricarinatus)雄性生殖系统各组织的生化测定和乳酸脱氢酶(LDH)、琥珀酸脱氢酶(SDH)、延胡索酸还原酶(FR)的活性测定发现,红螯螯虾精荚(含精子)中的糖类和蛋白质含量丰富,分别为18.264和12.846 mg/g(FW),总脂含量相对较低,仅为1.613 mg/g(FW);精子(含初级精荚壁)亦以糖类和蛋白质含量较高,分别达到5.227和8.475 mg/g(FW),总脂含量因过低而未能测出,但精子的上述物质含量均显著低于精荚。酶活性测定发现,LDH酶活性以精巢、输精管和射精管较高,其中又以前输精管最高,为1 426.258 U/(g protein),可能与其功能相关;精子和精荚LDH活性极显著低于输精管,含量分别为87.375和36.215 U/(g protein),两者间差异显著;FR活性在精荚和精子中较高,两者分别达到19.945和15.732 U/(mg protein),显著高于生殖系统其他组织器官,但均未检测到SDH酶活性,故其SDH/FR的比值趋向于0。而生化物质的组织化学以及酶组化定位的结果进一步证实了上述结果。此外,LDH同工酶酶谱结果证实,精荚仅含有一条厌氧型LDHA编码亚基占优势的同工酶谱带。所有实验结果表明,红螯螯虾精子代谢的方式以厌氧型为主,其代谢底物主要为糖类,为厌氧型糖酵解,其代谢底物由次级精荚壁提供。
Through the biochemical determination of the tissues of the male reproductive system of Cherax quadricarinatus and the activity determination of lactate dehydrogenase (LDH), succinate dehydrogenase (SDH) and fumarate reductase (FR) The content of carbohydrate and protein in Shrimp spp (sperm) was 18.264 and 12.846 mg / g (FW), the total lipid content was relatively low (1.613 mg / g FW) Pod wall) also had higher carbohydrate and protein contents, reaching 5.227 and 8.475 mg / g (FW), respectively. However, the total lipid content was underestimated, but the sperm content was significantly lower than that of the sperm. The activity of LDH was found to be higher in testis, vas deferens and ejaculatory ducts, of which 1426.258 U / (g protein) was the highest before the vas deferens, which may be related to its function. LDH activity of sperm and sperm was significantly lower than that of vas deferens , Respectively, which were 87.375 and 36.215 U / g protein, respectively. There was significant difference between them. The activity of FR was higher in sperm and sperm, reaching 19.945 and 15.732 U / mg respectively, which was significantly higher than that of reproductive system Other tissues and organs, but did not detect SDH activity, so its SDH / FR ratio tends to 0. The results of biochemical histochemistry and enzyme histochemistry localization confirm these results. In addition, LDH isozyme zymography confirmed that the spermatophore contains only one anaerobic LDHA coding subunit of the dominant isozyme bands. All the experimental results show that the way of metabolism of crayfish is anaerobic, the main metabolic substrate is saccharide, which is anaerobic glycolysis, and the metabolic substrate is provided by the secondary sperm wall.