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利用4对AFLP引物对来自安徽石台、湖北大悟和河南桐柏的3个省沽油群体进行遗传多样性分析,共扩增出489条带,其中多态性条带460条,多态性百分比为93.99%。不同群体Nei’s基因多样性指数(H)和Shannon’s信息指数(I)变化范围分别为0.1920～0.2046和0.2937～0.3151,其中湖北大悟群体遗传多样性最高。物种水平和种群水平的H分别为0.2190和0.1964,群体内变异占总变异的89.68%,表明省沽油遗传变异主要存在于各群体内部。3个群体的平均遗传距离为0.0292,UPGMA聚类分析结果说明省沽油各群体间亲缘关系较近并和地域具有相关性。建议省沽油的遗传资源保护应以种内遗传多样性的保护为主。
Four pairs of AFLP primers were used to analyze the genetic diversity of three provinces from Shitai of Anhui Province, Dawu of Hubei Province and Tongbai of Henan Province. A total of 489 bands were amplified, of which 460 polymorphic bands were polymorphic The percentage is 93.99%. The diversity index (H) and Shannon’s information index (I) of Nei’s from different populations ranged from 0.1920 to 0.2046 and from 0.2937 to 0.3151, respectively. Among them, the populations of Dawu in Hubei Province had the highest genetic diversity. H of species level and population level were 0.2190 and 0.1964, respectively, and intraspecies variation accounted for 89.68% of the total variation, indicating that the genetic variation of oilseed stocks mainly existed within each group. The average genetic distance of three populations was 0.0292. The results of UPGMA clustering analysis showed that the genetic relationship among the groups was close and related to the region. It is suggested that the protection of genetic resources for provincial oil sales should be mainly based on the protection of intraspecific genetic diversity.