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以控制拟南芥硫苷生物合成相关基因家族FMO GS-OXs全长cds为参考序列,在萝卜ESTs数据库中进行blastn检索,得到具备Score≥100、E-value≤10-10和coverage≥70%条件的24条萝卜ESTs作为候选的拟南芥FMO GS-OXs基因的同源ESTs,并进行序列聚类拼接、注释及分析。结果显示:在萝卜中发现两个旁系同源基因(RsFMO GS-OXa和RsFMO GS-OXb)。RsFMO GS-OXs与拟南芥FMO GS-OXs间在核酸水平上的一致性为68.0%~83.2%;RsFMO GS-OXs旁系同源基因间的一致性仅为67.3%;RsFMO GS-OXs与白菜FMO GS-OXs同源基因间的一致性为67.3%~92.9%。系统进化分析发现,RsFMO GS-OXa和白菜FMO GS-OX2基因亲缘关系最近,而RsFMO GS-OXb和白菜FMO GS-OX5a亲缘关系更近一些,同时认为,RsFMO GS-OXa和RsFMO GS-OXb的直系同源基因分别为FMO GS-OX2和FMO GS-OX5。
To control the Arabidopsis thaliana glucosinolate biosynthesis related gene family FMO GS-OXs full-length cds as a reference sequence, blastn search in the radish ESTs database to obtain with Score≥100, E-value≤10-10 and coverage≥70% Twenty-four radish ESTs were selected as homologous ESTs of the candidate Arabidopsis FMO GS-OXs genes, and the sequences were clustered, annotated and analyzed. The results showed that two paralogs (RsFMO GS-OXa and RsFMO GS-OXb) were found in radish. The consistency of RsFMO GS-OXs with Arabidopsis FMO GS-OXs at nucleotide level was 68.0% -83.2%; the identity of the paralogs between RsFMO GS-OXs was only 67.3%; RsFMO GS-OXs was The consistency of FMO GS-OXs homologous genes in Chinese cabbage was 67.3% -92.9%. Phylogenetic analysis showed that the genetic relationship between RsFMO GS-OXa and Chinese cabbage FMO GS-OX2 gene was the closest, while the relationship between RsFMO GS-OXb and Chinese cabbage FMO GS-OX5a was closer. At the same time, RsFMO GS-OXa and RsFMO GS-OXb Orthologous genes were FMO GS-OX2 and FMO GS-OX5, respectively.