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[目的]探讨高能X线和人参皂甙Rb1对人脑胶质瘤细胞株SHG-44的影响。[方法]应用MTT法检测细胞增殖情况;流式细胞仪检测细胞周期的变化;RT-PCR法检测MMP-2、MMP-9mRNA的表达水平。[结果]高能X线照射后SHG-44细胞增殖受到抑制,且随照射剂量加大抑制作用增强。当加入人参皂甙Rb1后,细胞增殖抑制更加明显;高能X线照射后SHG-44细胞表现为G2/M期阻滞,而人参皂甙Rb1可缓解G2/M期阻滞现象;辐射促进MMP-2、MMP-9的表达,且随照射剂量的增加表达量增加,而人参皂甙Rb1抑制其表达,与药物浓度呈反比。[结论]人参皂甙Rb1与高能X线协同抑制SHG-44的增殖,辐射后肿瘤细胞侵袭能力增强,而人参皂甙Rb1使照射后肿瘤细胞侵袭能力下降。
[Objective] To investigate the effects of high-energy X-ray and ginsenoside Rb1 on human glioma cell line SHG-44. [Methods] Cell proliferation was detected by MTT assay. Cell cycle was detected by flow cytometry. The expression of MMP-2 and MMP-9 mRNA was detected by RT-PCR. [Result] The proliferation of SHG-44 cells was inhibited after high-energy X-ray irradiation, and the inhibition was enhanced with the increase of irradiation dose. When Ginsenoside Rb1 was added, the cell proliferation was inhibited more significantly. SHG-44 cells showed G2 / M arrest after high-energy X-ray irradiation, while Ginsenoside Rb1 could alleviate G2 / M arrest. Radiation promoted MMP-2 , And the expression of MMP-9 increased with the increase of irradiation dose, while ginsenoside Rb1 inhibited its expression, which was inversely proportional to the drug concentration. [Conclusion] Ginsenoside Rb1 synergistically inhibits the proliferation of SHG-44 cells with high-energy X-ray irradiation and enhances the invasive ability of tumor cells after irradiation. However, ginsenoside Rb1 decreases the invasiveness of tumor cells after irradiation.