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利用静电纺丝技术制备了有序和无序聚己内酯(PCL)纤维支架。通过扫描电镜(SEM)、X射线衍射(XRD)和万能试验机对支架结构与形态、结晶性能及力学性能进行了表征;将体外培养的细胞接种至支架表面,用激光共聚焦显微镜(LSCM)和SEM观察了细胞在支架表面的生长情况。研究结果表明,电纺丝得到的纤维直径均一,有序化纤维支架中86.14%的纤维沿着确定方向有序排列;有序纤维拉伸强度为(27.63±0.75)MPa,比无序纤维的拉伸强度增大了232%。弹性模量基本不变,断裂伸长率明显降低;有序纤维对细胞的定向生长具有良好的引导作用。
The ordered and disordered polycaprolactone (PCL) fiber scaffolds were prepared by electrospinning. The structure and morphology, crystallinity and mechanical properties of scaffolds were characterized by scanning electron microscopy (SEM), X-ray diffraction (XRD) and universal testing machine. Cells cultured in vitro were seeded onto scaffolds and analyzed by laser confocal microscopy (LSCM) The growth of cells on the surface of the scaffolds was observed with SEM. The results showed that the fibers obtained by electrospinning were uniform in diameter and the order of 86.14% of the fibers in the ordered fiber scaffolds were arranged in a definite direction. The tensile strength of ordered fibers was (27.63 ± 0.75) MPa, which was higher than that of disordered fibers Tensile strength increased by 232%. Elastic modulus is basically unchanged, the elongation at break significantly reduced; ordered fibers on the directional growth of cells has a good lead.