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自1962年Wolf和Quimby首次建立RTG-2细胞系以来,鱼类细胞培养技术有了很大进展。据Wolf和Mann1980年的统计已有17属36种鱼61个硬骨鱼类细胞系相继建立。我国这方面工作起步较晚,主要是近10年的工作,但已建立了草鱼吻端组织细胞株(1981,张念慈杨广智),草鱼肾组织细胞系(1986,左文功等),草鱼尾鳍组织二倍体细胞系(1986,魏彦章等),草鱼吻端成纤维细胞系(1988,李焕林等)十余种细胞株(系)。建立细胞株(系)的目的旨在运用这一技术进行其它领域研究。从已发表的文献来看,鱼类细胞培养技术已用于鱼类遗传育种,鱼病防治,鱼类生理学,环境保护等领域的研究。本文就此作一概述。
Since 1962 Wolf and Quimby first established RTG-2 cell line, fish cell culture technology has made great progress. According to Wolf and Mann’s 1980 survey, 61 teleost fish cell lines belonging to 17 genera and 36 species have been established. Our work in this area started late, mainly for nearly 10 years of work, but the grass carp has established a tissue bank of the salivary glands (1981, Zhang Nianci Yang Guangzhi), grass carp kidney tissue cell line (1986, Zuobong Gong, etc.), grass carp caudal fin tissue Diploid cell line (1986, Wei Yanzhang, etc.), grass carp kindeye fibroblast cell line (1988, Li Huanlin, etc.) more than ten kinds of cell lines (lines). The purpose of establishing cell lines (lines) is to apply this technique to other areas of research. From the published literature, fish cell culture technology has been used in fish genetics and breeding, fish disease prevention and control, fish physiology, environmental protection and other fields. This article gives an overview of this.