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目的 :应用原子力显微镜检查术 (atomicforcemicroscopy,AFM)对电磁脉冲辐照前后培养的下丘脑神经细胞膜进行观察 ,以探讨电磁脉冲对其细胞膜影响的直接证据。方法 :对新生的Wistar乳大鼠下丘脑神经细胞在 6孔板中进行原代培养 ,在培养 14d时 ,用高场强电磁脉冲模拟源 (场强为 6× 10 4 V m ,脉冲上升时间 2 0ns,脉宽 30 μs)以 2 .5次 min ,辐照 2min。辐照后即刻固定细胞 ,应用AFM对细胞表面进行接触式连续扫描。结果 :电磁脉冲辐照后即刻就可引起下丘脑神经细胞膜表面大小不一、深度不同、类圆形和不规则形的穿孔 ,穿孔最大直径为 2 73.2 1～330 .10nm ,深度达 13.5 7nm。结论 :电磁脉冲辐照后可直接导致下丘脑神经元细胞膜的穿孔 ,提示其可能是电磁脉冲生物效应的损伤机制之一。
OBJECTIVE: To observe the hypothalamic neuronal membranes cultured before and after electromagnetic pulse irradiation by atomic force microscopy (AFM) to explore the direct evidence of the effect of electromagnetic pulse on the cell membrane. Methods: Hypothalamic neurons of newborn Wistar rats were primarily cultured in 6-well plates. After culturing for 14 days, high-strength electromagnetic pulse simulation source (field intensity 6 × 10 4 V m, pulse rise time 2 0ns, pulse width 30 μs) to 2.5 times min, irradiation 2min. Cells were fixed immediately after irradiation and AFM was used to scan the cell surface continuously. Results: Immediately after irradiation of electromagnetic pulse, the membrane surface of the hypothalamic neurons could cause different sizes, depths, round and irregular perforations. The maximum diameter of perforation was 2 73.2 1 ~ 330 .10 nm and the depth was 13.5 7 nm. Conclusion: The irradiation of electromagnetic pulse can directly lead to the perforation of the cell membrane of hypothalamic neurons, suggesting that it may be one of the mechanisms of biological effects of electromagnetic pulse.